The amount of rainfall and its distribution in time and space is dependent on the atmospheric instability conditions, and on its moisture content. The aim of this study was to determine the atmospheric instability conditions during January to March (JFM), February to April (FMA), March to May (MAM), and October to December (OND) rainy seasons over local climate zones in Tanzania. Zone area average seasonal Convective Available Potential Energy (CAPE), Convective Inhibition (CIN), Precipitable Water (PW) and Lifted index (Li) were calculated and analyzed. Results showed Li < 0 in JFM and FMA over whole Tanzania. During MAM and OND, Li < 0 over the Lake Zone, Western Highlands Zone and Central Zone only. CAPE ranged from 793 J/kg to 1183 J/kg during JFM, and 700 J/kg to 1080 J/kg during FMA. During MAM, CAPE ranged from 170 J/kg to 921 J/kg and from 173 J/kg to 833 J/kg during OND. Results also showed CAPE > 1000 J/kg over the Lake Zone, Western Highlands Zone, Island Zone, and Central Zone. These results show that the atmosphere was moderately unstable during the JFM and FMA and was weakly unstable during the MAM and OND. Therefore, the atmosphere is likely to be more convective during JFM and FMA seasons. Keywords: Lifted index, Convective inhibition, Precipitable water, Convective available potential energy, Atmospheric instability.