Introduction: Organ donation deals with the surgery which includes harvesting the organ and transplants them into end stage organ disease patient which on progression required organ transplantation surgeries. Organ donation rate in India is only 0.26 per million. 1 Absence of consciousness about the requirement of organ donation and the unfamiliarity among medical fraternity regarding its significance knowledge and legal issues create the huge splintering for end stage organ disease Patient. The objective of this study is to assess the awareness, knowledge, attitude and practice regarding organ donation among the Medical Teachers, & students perusing Post-Graduation. Materials and methods: Study was conducted among 100 subjects, where 50 Medical Teachers & 50 students pursuing Post-Graduation have participated. Questionnaires are distributed in order to assess their awareness, knowledge, attitude and practice regarding organ donation among the Medical Teachers, & students perusing Post-Graduation. Collected Data was analysed using Microsoft Excel program. Results: Total 100 subjects participated in this study, among this 50 are Medical Teachers & 50 are student pursuing post-graduation. Only 33% have gone through the provisions of Transplantation of human organ & tissue act, 1994, although 68% among them have motivated the patient's relative for the organ donation. Conclusion: Requirement for organ donation have been showing tremendous upsurge trends as there are increased number of patients diagnosed with end stage organ disease. On the other side lack of awareness, knowledge, attitude and practice regarding organ donation shall hamper the current scenario for upgradation of health status among end stage organ disease patient.