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Tropilaelaps mercedesae parasitism can cause Apis mellifera colony mortality in Asia. Here, we report for the first time that tropilaelaps mites feed on both pre- and post-capped stages of honey bees. Feeding on pre-capped brood may extend their survival outside capped brood cells, especially in areas where brood production is year-round. In this study, we examined the types of injury inflicted by tropilaelaps mites on different stages of honey bees, the survival of adult honey bees, and level of honey bee viruses in 4th instar larvae and prepupae. The injuries inflicted on different developing honey bee stages were visualised by staining with trypan blue. Among pre-capped stages, 4th instar larvae sustained the highest number of wounds (4.6 ± 0.5/larva) while 2nd-3rd larval instars had at least two wounds. Consequently, wounds were evident on uninfested capped brood (5th-6th instar larvae = 3.91 ± 0.64 wounds; prepupae = 5.25 ± 0.73 wounds). Tropilaelaps mite infestations resulted in 3.4- and 6-fold increases in the number of wounds in 5th-6th instar larvae and prepupae as compared to uninfested capped brood, respectively. When wound-inflicted prepupae metamorphosed to white-eyed pupae, all wound scars disappeared with the exuviae. This healing of wounds contributed to the reduction of the number of wounds (≤10) observed on the different pupal stages. Transmission of mite-borne virus such as Deformed Wing Virus (DWV) was also enhanced by mites feeding on early larval stages. DWV and Black Queen Cell Virus (BQCV) were detected in all 4th instar larvae and prepupae analysed. However, viral levels were more pronounced in scarred 4th instar larvae and infested prepupae. The remarkably high numbers of wounds and viral load on scarred or infested developing honey bees may have caused significant weight loss and extensive injuries observed on the abdomen, wings, legs, proboscis and antennae of adult honey bees. Together, the survival of infested honey bees was significantly compromised. This study demonstrates the ability of tropilaelaps mites to inflict profound damage on A. mellifera hosts. Effective management approaches need to be developed to mitigate tropilaelaps mite problems.

The Asian honey bee (Apis dorsata) is distinct from its more widely distributed cousin Apis mellifera by a few key characteristics. Most prominently, A. dorsata, nest in the open by forming a colony clustered around the honeycomb, whereas A. mellifera nest in concealed cavities. Additionally, the worker and reproductive castes are all of the same size in A. dorsata. In order to investigate these differences, we performed whole genome sequencing of A. dorsata using a hybrid Oxford Nanopore and Illumina approach. The 223 Mb genome has an N50 of 35 kb with the largest scaffold of 302 kb. We have found that there are many genes in the dorsata genome that are distinct from other hymenoptera and also large amounts of transposable elements, and we suggest some candidate genes for A. dorsata’s exceptional level of defensive aggression.

Edible insects have become increasingly popular in Thailand as a nutritious and appealing alternative food source. As the edible insect industry in the country expands rapidly, efforts are being made to transform it into an economically viable sector with substantial commercial potential. Some of the most consumed and sold edible insects in Thailand include locusts, palm weevils, silkworm pupae, bamboo caterpillars, crickets, red ants, and giant water bugs. With its strong growth, Thailand has the potential to emerge as a global leader in the production and promotion of edible insect products. Edible insects are an excellent source of protein, fat, vitamins, and minerals. In particular, crickets and grasshoppers are protein-rich, with the average protein content of edible insects ranging from 35 to 60 g/100 g of dry weight or 10 to 25 g/100 g of fresh weight. This surpasses the protein content of many plant-based sources. However, the hard exoskeleton of insects, which is high in chitin, can make them difficult to digest. In addition to their nutritional value, edible insects contain biologically active compounds that offer various health benefits. These include antibacterial, anti-inflammatory, anti-collagenase, elastase-inhibitory, α-glucosidase-inhibitory, pancreatic lipase-inhibitory, antidiabetic/insulin-like/insulin-like peptide (ApILP), antidiabetic, anti-aging, and immune-enhancing properties. The Thai food industry can process and utilize edible insects in diverse ways, such as low-temperature processing, including refrigeration and freezing, traditional processing techniques, and incorporating insects into products, such as flour, protein, oil, and canned food. This review offers a comprehensive overview of the status, functional properties, processing, and utilization of edible insects in Thailand, and it serves as a valuable resource for those interested in edible insects and provides guidance for their application in various fields.

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