No abstract

Combined Nd:YAG laser and topical fluoride treatment was effective for reducing the incidence of caries in vivo.

RESUMO: Um Banco de Dentes Humanos (BDH) é uma instituição sem fins lucrativos, vinculada a uma faculdade, universidade ou outra instituição. Seu propósito é suprir as necessidades acadêmicas, fornecendo dentes humanos para pesquisa ou para treinamento laboratorial pré-clínico dos alunos, dessa forma eliminando o comércio ilegal de dentes que ainda existe nas faculdades de Odontologia. Cabe também ao BDH zelar pela eliminação da infecção cruzada que existe no manuseio indiscriminado de dentes extraídos. Para o bom funcionamento de um BDH, é fun damen tal um controle severo de seus procedimentos internos, que incluem a separação e o estoque de dentes, assim como o cadastro e o arquivamento das fichas dos doadores ou beneficiários. Aqui, abordaremos as funções que um BDH pode desempenhar e a forma pela qual hoje o Banco de Dentes Humanos da Faculdade de Odontologia da Universidade de São Paulo (FOUSP) funciona e organiza-se. DESCRITORES:Ban co de den tes hu ma nos; Orga ni za ção e ad mi nis tra ção.ABSTRACT: A Hu man Te eth Bank (HTB) is a non pro fit ins ti tu ti on, as so ci a ted to a col le ge, to a u ni ver sity or to some other ins ti tu ti on. Its pur po se is to ful fill aca de mic ne eds, by sup plying hu man te eth for re se arch or for pre cli ni cal la bo ra tory tra i ning of stu dents, thus eli mi na ting the il le gal com mer ce of te eth that still ta kes pla ce in den tal scho ols. It is also up to an HTB to work for the eli mi na ti on of cross-in fec ti on ca u sed by in dis cri mi na te hand ling of ex trac ted te eth. In or der to work pro perly, an HTB should have a strict con trol of its in ter nal pro ce du res, which in c lu de se pa ra ti on and stoc king of te eth, as well as an ap pro pri a te ma in ta i ning of do nors' and be ne fi ci a ri es' re cords. In this ar ti cle, we dis cuss the func ti ons that an HTB can per form, and how the Hu man Te eth Bank, Scho ol of Den tistry, Uni ver sity of São Pa u lo, works and or ga ni zes it self to day. DESCRIPTORS:Hu man teeth bank; Or ga ni za tion and ad min is tra tion.Um Ban co de Den tes Hu ma nos (BDH) é uma ins ti tu i ção sem fins lu cra ti vos, que deve es tar vincu la da a uma fa cul da de, uni ver si da de ou ou tra ins ti tu i ção. Seu pro pó si to é su prir as ne ces si da des aca dê mi cas, for ne cen do den tes hu ma nos para pes qui sa ou ati vi da des di dá ti cas.Den tro des se con tex to, a ins ti tu i ção BDH pas sa a as su mir im por tan te fun ção éti ca, eli mi nan do o co mér cio ile gal de den tes que ain da exis te nas facul da des de Odon to lo gia. De fato, o den te é um ór -gão do cor po hu ma no e, como tal, está sub me ti do à Lei de Trans plan tes Bra si le i ra (lei 9434 de 04/02/1997), a qual pre vê pena de 3 a 8 anos de re clu são e mul ta para quem re mo ver, post-mor tem, ór gãos, te ci dos e par tes do cor po hu ma no de pesso as não iden ti fi ca das. O Có di go Pe nal tam bém pre vê pena de 1 a 3 anos de re clu são para aque les que vi o la rem se pul tu ra (Arti go 210) e o Con se lho Na ci o nal de ...

This study evaluated the resistance to demineralization and fluoride incorporation of enamel irradiated with Er:YAG. A total of 110 bovine teeth were selected and divided into eight groups: unlased, 37% phosphoric acid, and samples irradiated with the Er:YAG laser at several fluences (31.84 J/cm(2), 25.47 J/cm(2), 19.10 J/cm(2), 2.08 J/cm(2), 1.8 J/cm(2), and 0.9 J/cm(2)). The application of acidulated phosphate fluoride was performed after treatments. All samples were immersed in 2 ml of 2.0 M acetic-acetate acid solution at pH 4.5 for 8 h, and fluoride, calcium, and phosphorus ions dissolved were analyzed by atomic absorption spectrometry and spectrophotometry. The phosphoric acid and 31.84 J/cm(2) groups presented the lowest dissolution of calcium and phosphorus ions. Higher fluoride incorporation was observed on 1.8 J/cm(2) and 0.9 J/cm(2) groups. Based on these results, Er:YAG laser was able to decrease acid dissolution and increase fluoride uptake and can be a promissory alternative for preventive dentistry.

This study evaluated the effect of Er,Cr:YSGG laser irradiation and professional fluoride application on enamel demineralization and on fluoride formation and retention.In a blind in vitro study, 264 human enamel slabs were distributed into 8 groups: G1 – untreated; G2 – treated with acidulated phosphate fluoride gel (APF gel, 1.23% F) for 4 min; G3, G4 and G5 – irradiated with Er,Cr:YSGG at 2.8, 5.6 and 8.5 J/cm², respectively; G6, G7 and G8 – preirradiated with Er,Cr:YSGG at 2.8, 5.6 and 8.5 J/cm², respectively, and subjected to APF gel application. Twenty slabs of each group were submitted to a pH-cycling regimen, and enamel demineralization was evaluated in 10 slabs of each group. In the other 10 slabs, CaF₂-like material was determined. To evaluate F formed, 10 additional slabs of each group, not subjected to the pH cycling, were submitted to analysis of CaF₂-like material and fluorapatite, while the other 3 slabs of each group were evaluated by scanning electron microscopy. The F content was also measured in all pH-cycling solutions. Laser at 8.5 J/cm² and APF treatment reduced enamel demineralization compared to the control (p < 0.05), but the combination of these treatments was not more efficient than their isolated effect. A higher concentration of retained CaF₂-like material was found in laser groups followed by APF in comparison with the APF gel treatment group. The findings suggest that laser treatment at 8.5 J/cm² was able to decrease hardness loss, even though no additive effect with APF was observed. In addition, laser treatment increased the formation and retention of CaF₂ on dental enamel.