No abstract

In recent studies (Tarshis, 1952, 1953) it was found that a modified human blood medium was more satisfactory for the cultivation of tubercle bacilli than several other blood media previously described (Tarshis and Frisch, 1951; Frisch and Tarshis, 1951). Since comparison of this medium under routine diagnostic condition was somewhat limited, a more thorough evaluation was felt desirable. In this investigation the blood medium was compared with the Lowenstein-Jensen medium (Holm and Lester, 1941, 1947). MATERIALS AND METHODS The formulas and the methods of preparing the media are given below. Blood medium. Agar,' 1.5 g; glycerol (analytical grade), 1.0 ml; human bank blood (with ACD solution, see below), 25.0 ml; water (distilled), 74.0 ml; penicillin (50 units per ml)2; final pH approximately 6.8.'