Biodiesel wash water is a contaminating industrial effluent that must be treated prior to disposal. The use of this effluent as a low-cost alternative cultivation medium for microalgae could represent a viable supplementary treatment. We cultivated 11 microalgae species with potential use for biodiesel production to assess their growth capacities in biodiesel industrial washing waters. Only Monoraphidium contortum, Ankistrodesmus sp., Chlorococcum sp., and one unidentified Chlorophyceae species grew effectively in that effluent. M. contortum showed the highest growth capacity and had the second highest fatty acid content (267.9 mg g of DW), predominantly producing palmitic (20.9%), 7,10,13-hexadecatrienoic (14%), oleic (16.2%), linoleic (10.5%), and linolenic acids (23.2%). In the second phase of the experiment, the microalgae were cultivated in biodiesel wash water at 75% of its initial concentration as well as in WC (control) medium. After 21 days of cultivation, 25.8 and 7.2% of the effluent nitrate and phosphate were removed, respectively, and the chemical oxygen demand was diminished by 31.2%. These results suggest the possibility of cultivating biodiesel producing microalgae in industrial wash water effluents.
Shrimp farm effluents are one of the principal causes of eutrophication in coastal environments. Integrated processes of bioremediation involving the culturing of purifying organisms have been suggested, but very few studies have focused on microalgae. For that purpose evaluated the growth potential of Amphora sp. in the residual waters of shrimp farm activity fulfilled on the Paraíba State, Brazil. The experiments were performed using Conway medium as the control and wastewaters from shrimp farm at 100% concentrations. Amphora sp. demonstrated good growth in the shrimp farm effluents under test conditions, although less than that observed in the control medium. This diatom was found to removed 73.357 and 72.572% of PO and NO, respectively, demonstrating a high mitigation potential for this type of effluent. Comparisons of the physiological responses measured by flow cytometry demonstrated higher cell densities of Amphora sp. in the control medium, but a higher lipid content was observed in Amphora cultured in shrimp farm effluents.
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