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This chapter presents a collection of studies performed in the Amazon region that includes thematic products portraying its fluvial sensitivity to oil spills. The research addresses the intense Amazonian seasonal dynamics, as well as the environmental peculiarities of this singular ecosystem. Periodic changes caused by natural phenomena have a significant impact on not only flooded alluvial plains and riverine habitats but also on petroleum exploration, production, and transportation activities. Therefore, the implementation of tools to assess the potential impact of oil spills in the Amazonian rivers must be adjusted to the local conditions. The main deliverables of the research are (1) fluvial oil spill sensitivity index maps contemplating each phase of the hydrological cycle (low water, high water, receding water, and rising water), (2) a computational method to represent fluctuations of the seasonal inundation, and (3) a risk analysis method using linguistic rules for the construction of a risk matrix.