ResumoEste estudo teve como objetivo verificar a ocorrência de microorganismos isolados de pacientes hospitalizados durante as etapas de implantação da Comissão AbstractThis study aimed at verifying the occurrence of isolated microorganisms of hospitalized patients during the stages of implantation of the Hospital Infection Control Commission (HICC) in an Intensive Care Unit at a private hospital in the city of Toledo, Paraná. It is about a retrospective study, during the period of February/ 2007 to March/ 2008. Of the total internments, 37 patients presented positive cultures and Pseudomonas aeruginosa was the most isolated microorganism, with 30.77% incidence, followed by Staphylococcus epidermidis, 23.08%, followed by other seven microorganisms with inferior incidences.