Purpose: the objective of the work is to determine the testicular regions favorable for the realization of a testicular biopsy without damaging the important vessels. Material and Methods: 8 left and right testes were collected from 3 immature Wistar rats and 1 adult control, aged 15, 25 and 40 days for the young rats and 7 months for the adult. The testes were taken under inhalation anesthesia. The testes were examined by eye and the images were obtained from a digital camera. Results: Results showed that the testicular (or spermatic) artery with a large caliber had a subcapsular arrangement. It was tortuous and followed the anterior edge of the testicle from the upper pole to the lower pole. As that vessel passed along this place it formed an arterial handle and ascended along the posterior edge. The arrangement of this artery was identical for all populations of rats. Very small vessels were observed beneath the internal surface of the albuginea of the testis mainly from the lateral areas of the testis. They had transverse, horizontal and oblique directions and which intensified with the age of the animals. Conclusion: Anatomically, the results of this study highlight the situation and caliber variability of the superficial vessels of the testis and the importance of the testicular artery. Clinically, they could be useful for testicular biopsy, we suggest that it be practiced away from the large vessels, that is to say in the lateral areas of the testicle.