Process-based hydrologic models allow to identify the behavior of a basin providing a mathematical description of the hydrologic processes underlying the runoff mechanisms that govern the streamflow generation. This study focuses on a macroscale application of the Variable Infiltration Capacity (VIC) model over 31 headwater subwatersheds belonging to the Duero River Basin, located in the Iberian Peninsula, through a threepart approach: (1) the calibration and validation of the VIC model for all the subwatersheds; (2) an integrated sensitivity analysis concerning the soil parameters chosen for the calibration, and (3) an assessment of equifinality and the efficiency of the calibration algorithm. The calibration and validation processes showed good results for most of the subwatersheds in a computationally efficient way using the Shuffled-Complex-Evolution algorithm. The sensitivity measures were obtained with the Standardized Regression Coefficients method through a post-process of the outputs of a Monte Carlo simulation carried out for 10 000 parameter samples for each subwatershed. This allowed to quantify the sensitivity of the water balance components

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Monthly streamflow records from a set of gauging stations, selected to form a reference hydrologic network, are analyzed together with precipitation and temperature data to establish whether the streamflows in the Guadalquivir River Basin have experienced changes during the last half of the XXth century that can be attributed to hydrological forcing. The observed streamflows in the reference network have undergone generalized and significant decreases both at seasonal and annual scales during the study period. Annual rainfall, though, did not experienced statistically

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