Acinetobacter are a major concern because of their rapid development of resistance to a wide range of antimicrobials, and rapid profundity in transformation, surviving desiccation and persistinge in the environment for a very long time. The organisms are associated with bacteraemia, pulmonary infections, meningitis, diarrhea and notorious nosocomial infections with mortality rates of 20 to 60%. Transmission is via person-to-person contact, water and food contamination, and contaminated hospital equipment. The increasing virulence and rapid development of multidrug resistance by these organisms highlights the need to search for alternatives for chemotherapy. A poor understanding of the organisms and dearth of information about their occurrence especially in developing countries informed the need for this review paper.

Plants with pesticidal properties have been investigated for decades as alternatives to synthetics, but most progress has been shown in the laboratory. Consequently, research on pesticidal plants is failing to address gaps in our knowledge that constrain their uptake. Some of these gaps are their evaluation of their efficacy under field conditions, their economic viability and impact on beneficial organisms. Extracts made from four abundant weed species found in northern Tanzania, Tithonia diversifolia, Tephrosia vogelii, Vernonia amygdalina and Lippia javanica offered effective control of key pest species on common bean plants (Phaseolus vulgaris) that was comparable to the pyrethroid synthetic, Karate. The plant pesticide treatments had significantly lower effects on natural enemies (lady beetles and spiders). Plant pesticide treatments were more cost effective to use than the synthetic pesticide where the marginal rate of return for the synthetic was no different from the untreated control, around 4USD/ha, compared to a rate of return of around 5.50USD/ha for plant pesticide treatments. Chemical analysis confirmed the presence of known insecticidal compounds in water extracts of T. vogelii (the rotenoid deguelin) and T. diversifolia (the sesquiterpene lactone tagitinin A). Sesquiterpene lactones and the saponin vernonioside C were also identified in organic extracts of V. amygdalina but only the saponin was recorded in water extracts which are similar to those used in the field trial. Pesticidal plants were better able to facilitate ecosystem services whilst effectively managing pests. The labour costs of collecting and processing abundant plants near farm land were less than the cost of purchasing synthetic pesticides.

In the fight against arthropod crop pests using plant secondary metabolites, most research has focussed on the identification of bioactive molecules. Several hundred candidate plant species and compounds are now known to have pesticidal properties against a range of arthropod pest species. Despite this growing body of research, few natural products are commercialized for pest management whilst on-farm use of existing botanically-based pesticides remains a small, but growing, component of crop protection practice. Uptake of natural pesticides is at least partly constrained by limited data on the trade-offs of their use on farm. The research presented here assessed the potential trade-offs of using pesticidal plant extracts on legume crop yields and the regulating ecosystem services of natural pests enemies. The application of six established pesticidal plants (Bidens pilosa, Lantana camara, Lippia javanica, Tephrosia vogelii, Tithonia diversifolia, and Vernonia amygdalina) were compared to positive and negative controls for their impact on yields of bean (Phaseolus vulgaris), cowpea (Vigna unguiculata), and pigeon pea (Cajanus cajan) crops and the abundance of key indicator pest and predatory arthropod species. Analysis of field trials showed that pesticidal plant treatments often resulted in crop yields that were comparable to the use of a synthetic pesticide (lambda-cyhalothrin). The best-performing plant species were T. vogelii, T. diversifolia, and L. javanica. The abundance of pests was very low when using the synthetic pesticide, whilst the plant extracts generally had a higher number of pests than the synthetic but lower numbers than observed on the negative controls. Beneficial arthropod numbers were low with synthetic treated crops, whereas the pesticidal plant treatments appeared to have little effect on beneficials when compared to the negative controls. The outcomes of this research suggest that using extracts of pesticidal plants to control pests can be as effective as synthetic insecticides in terms of crop yields while tritrophic effects were reduced, conserving the non-target arthropods that provide important ecosystem services such as pollination and pest regulation. Thus managing crop pests using plant secondary metabolites can be more easily integrated in to agro-ecologically sustainable crop production systems.

On-farm experiments were conducted in farmers’ fields at 12 different sites in the 2 districts of Moshi and Rombo in northern Tanzania during the 2000–01 cropping season to study the effects of (brady)rhizobial inoculation in combination with P supply on growth and grain yields of soybean and common bean, and to assess the economic returns of these different technologies to farmers. A low level of N was included as an indicator of endogenous soil N status. The treatments included (brady)rhizobial inoculation, N fertilisation (30 kg N/ha as urea), P application [26 kg P/ha as triple super phosphate (TSP)], (brady)rhizobial inoculation + P fertilisation (26 kg/ha as TSP) and unfertilised uninoculated control. The study was conducted as a randomised complete block design with each of the 12 farmers’ fields as a replicate. At harvest, plant growth of soybean and common bean was significantly (P≤0.05) greater with (brady)rhizobial inoculation compared with N and P supply or uninoculated control in the 2 districts. Relative to uninoculated unfertilised plots, grain yields of common bean were markedly (P≤0.05) increased by 60–78% from inoculation alone, and 82–95% from inoculation + 26 kg P/ha; with soybean there was 127–139% increase in grain yield from inoculation alone, and 207–231% from inoculation + P. Thus, the combined application of bacterial inoculants and P fertiliser to field plants of soybean and common bean significantly (P≤0.05) increased biomass production and grain yield compared with the single use of N and P or (brady)rhizobial strains. From economic analysis, the increase in grain yield with inoculation translated into a significantly (P≤0.05) higher marginal rate of return and dollar profit for soybean and common bean farmers in northern Tanzania. With common bean, there was a 66 and 92% increase, respectively, in dollar profit with inoculation at Moshi and Rombo districts respectively relative to control; these profit margins rose to 84 and 102% with provision of supplemental P (26 kg P/ha). With soybean, however, the increase in profit with inoculation was much larger, about 140 and 153% at Rombo and Moshi, respectively, and these rose to 224 and 250% with P supply.