Underwater adhesion has numerous potential medical, household, and industrial applications. It is typically achieved through covalent polymerization and cross-linking reactions and/or the use of highly specialized biological or biomimetic polymers. As a simpler alternative to these covalent and biomimetic strategies, this article shows that stiff, gel-like complexes that adhere to various substrates under water can also be prepared through the ionic cross-linking of common, commercial polyelectrolytes. The gels form spontaneously when synthetic polycations, such as poly(allylamine) (PAH), are mixed with strongly binding multivalent anions, pyrophosphate (PPi) and tripolyphosphate (TPP). The PAH/PPi and PAH/TPP gels exhibit very high storage moduli (G∞′ ≈ 400 kPa), self-heal when torn, and adhere to both hydrophilic and hydrophobic substrates under water (with short-term tensile adhesion strengths of 350–450 kPa). Furthermore, these gels can be dissolved on demand (if adhesion needs to be reversed) by changing the ambient pH, which controls the ionization state of the polyelectrolyte and ionic cross-linker. These properties suggest that synthetic polycations cross-linked with PPi and TPP ions could provide a simple, inexpensive, and scalable platform for underwater adhesion.

Gel-like coacervates that adhere to both hydrophilic and hydrophobic substrates under water have recently been prepared by ionically cross-linking poly(allylamine) (PAH) with pyrophosphate (PPi) and tripolyphosphate (TPP). Among the many advantages of these underwater adhesives (which include their simple preparation and low cost) is their ability to dissolve on demand when exposed to high or low pH. To further analyze their stimulus-responsive properties, we have investigated the pH and ionic strength effects on the formation, rheology and adhesion of PAH/PPi and PAH/TPP complexes. The ionic cross-linker concentrations needed to form these adhesives decreased with increasing pH and ionic strength (although the complexes ceased to form when the parent solution pH exceeded ca. 8.5; i.e., the effective pKa of PAH). Once formed, their ionic cross-links were most stable (as inferred from their relaxation times) at near-neutral or slightly alkaline pH values (of roughly 6.5-9) and at low ionic strengths. The decrease in ionic cross-link stability within complexes prepared at other pH values and at elevated (150-300 mM) NaCl concentrations diminished both the strength and longevity of adhesion (although, under most conditions tested, the short-term tensile adhesion strengths remained above 10(5) Pa). Additionally, the sensitivity of PAH/PPi and PAH/TPP complexes to ionic strength was demonstrated as a potential route to injectable adhesive design (where spontaneous adhesive formation was triggered via injection of low-viscosity, colloidal PAH/TPP dispersions into phosphate buffered saline). Thus, while the sensitivity of ionically cross-linked PAH networks to pH and ionic strength can weaken their adhesion, it can also impart them with additional functionality, such as minimally invasive, injectable delivery, and ability to form and dissolve their bonds on demand.

Long-term (multiple-week or -month) release of small, water-soluble molecules from hydrogels remains a significant pharmaceutical challenge, which is typically overcome at the expense of more-complicated drug carrier designs. Such approaches are payload-specific and include covalent conjugation of drugs to base materials or incorporation of micro- and nanoparticles. As a simpler alternative, here we report a mild and simple method for achieving multiple-month release of small molecules from gel-like polymer networks. Densely cross-linked matrices were prepared through ionotropic gelation of poly(allylamine hydrochloride) (PAH) with either pyrophosphate (PPi) or tripolyphosphate (TPP), all of which are commonly available commercial molecules. The loading of model small molecules (Fast Green FCF and Rhodamine B dyes) within these polymer networks increases with the payload/network binding strength and with the PAH and payload concentrations used during encapsulation. Once loaded into the PAH/PPi and PAH/TPP ionic networks, only a few percent of the payload is released over multiple months. This extended release is achieved regardless of the payload/network binding strength and likely reflects the small hydrodynamic mesh size within the gel-like matrices. Furthermore, the PAH/TPP networks show promising in vitro cytocompatibility with model cells (human dermal fibroblasts), though slight cytotoxic effects were exhibited by the PAH/PPi networks. Taken together, the above findings suggest that PAH/PPi and (especially) PAH/TPP networks might be attractive materials for the multiple-month delivery of drugs and other active molecules (e.g., fragrances or disinfectants).

Soil analysis is a key practice to increase the efficiency of nutrient management in agriculture. Since the early 20th century, increasingly sophisticated methods have been developed to describe and manipulate the inherent spatial variability in soil chemical properties within the realms of classical and spatial statistics. In this paper, we reviewed design‐based (classical) and model‐based (geostatistical) sampling to suggest field‐scale sampling strategies consistent with common agronomic management goals in annual crop production systems. To assess the relevance of common sampling methods in relation to practice, current extension recommendations across the United States were compared with results from peer‐reviewed literature. Despite decades of research, specific recommendations for sample sizes, sampling depths, numbers of soil cores, and layouts were highly variable for classical and geostatistical approaches. Mobile nutrients, such as NO3, are frequently lacking in spatial structure and rarely are recommended for site‐specific management. Nonmobile nutrients, such as P, are more spatially dependent and exhibit nested spatial structures that are inconsistent across fields. For these reasons, we recommend design‐based sampling in most situations for simplicity, cost, and objectivity. The common design‐based sampling protocol prescribes collection of individual cores in a zig‐zag pattern that are combined to produce a composite sample. This protocol should be amended because it is not sufficiently randomized and is inadequate for log‐normally distributed variables. To facilitate site‐specific management, we recommend structured approaches for delineating management zones or strata and for researchers to systematically enumerate confounding variables while explicitly defining the scope of inference for future soil sampling studies.

No abstract