A Gram-stain-negative, coccus-shaped, aerobic bacterium, strain E6 T , was isolated from soil. 16S rRNA gene sequence analysis revealed a cluster containing strain E6 T and Paracoccus sediminis CMB17 T (the highest 16S rRNA gene similarity of 97.2 %). The other strains investigated showed 16S rRNA gene sequence similarities of less than 97 % to strain E6 T . The DNA-DNA relatedness between strain E6 T and P. sediminis DSM 26170 T was 59.2 %. Strain E6 T also shows some differences compared with other Paracoccus strains such as motility and inability to utilize lactate and propionate as sole carbon sources. The major fatty acids of strain E6 T were C 18 : 1 v7c and C 18 : 0 and ubiquinone-10 was the only respiratory quinone. Strain E6 T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unknown aminolipid and an unknown glycolipid as major polar lipids. The genomic DNA G+C content was 68.1 mol%. On the basis of phenotypic and genotypic characteristics, strain E6 T is concluded to represent a novel species of the genus Paracoccus, for which the name Paracoccus angustae sp. nov. is proposed. The type strain is E6 T (5KCTC 42473 T 5CCTCC AB 2015056 T ).The genus Paracoccus belongs to the family Rhodobacteraceae of the order Rhodobacterales in Proteobacteria. The genus Paracoccus comprises, at the time of writing, 40 recognized species and was first proposed by Davis et al. (1969) with Paracoccus denitrificans as the type species. Members of this genus have been isolated from various environments including soil (Dastager et al., 2011), bioreactor (La et al., 2005, sediment (Pan et al., 2014) and activated sludge (Lee & Lee, 2013;Sun et al., 2013). Members of the genus Paracoccus are Gram-stain-negative, are positive for oxidase and catalase, and have a high metabolic versatility, C 18 : 1 v7c as the major fatty acid and ubiquinone-10 (Q-10) as the predominant respiratory quinone (Dastager et al., 2011;Sheu et al., 2011). In addition, members of the genus have high DNA G+C contents and have phosphatidylglycerol and phosphatidylcholine as major polar lipids (Liu et al., 2008;Roh et al., 2009;Fu et al., 2011;Sheu et al., 2011; Kämpfer et al., 2012;Lee & Lee, 2013;Liu et al., 2013;Sun et al., 2013;Zhu et al., 2013;Pan et al., 2014;Zhang et al., 2014). Some strains can accumulate poly-b-hydroxybutyrate granules (Berry et al., 2003;Sheu et al., 2011;Lee & Lee, 2013;Pan et al., 2014). Strain E6 T was isolated from soil collected from Grand Canyon of Enshi City (1098 189 E 308 249 N, altitude about 1200 m), Hubei Province, China. The soil sample was serially diluted with 0.85 % (w/v) NaCl and incubated on 1/10 trypticase soy agar (TSA; Bacto) and incubated at 28 8C for 7 days. The purity of the colonies was checked by repeated streaking of single colonies on 1/10 TSA medium. The strain was preserved at 280 8C with 25 % (v/v) glycerol. The most closely related type strain, P. sediminis DSM 26170 T , and that of the type species of the genus, Paracoccus denitrificans DSM 413 T , were used as reference st...

No abstract