Cultures of Nitzschia palea were grown in a modified Chu 10 medium over a pH range from 4.0 to 9.9.No growth occurred at pH 4.7 or below, but there was increased cell yield at pH 6.4 to 9.9. Cell yield of the diatoms at pH 7 was less than at pH 8.2. Diatoms did not survive in strongly buffered media at 5.0 g/l or above, but did grow in media buffered at 0.63 g/l or less. Nitzschia palea appears to be alkaliphilous.
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