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This study evaluated effects of green compared with brown coating colors and varying levels of hydrolyzed squid peptide powder (HSP) on seafood flavor perception, sensory liking, emotions, and purchase intent (PI) of coated peanuts. Dried squid head was enzymatically hydrolyzed to produce HSP, which was then incorporated into peanut coating material at 0%, 0.89%, 1.78%, and 2.66% levels. Green‐coated peanuts (GCP) and brown‐coated peanuts (BCP) were produced and tested with U.S. consumers. A 2‐AC test (N = 100 consumers) was used to determine effects of coating colors on expected/perceived seafood flavor intensity of GCP compared with BCP at an equal HSP level based separately on looking, smelling, and tasting. Only tasting produced perceptual differences, at 1.78% and 2.66% HSP, with stronger seafood flavor intensity observed for GCP. Consumer testing (N = 160) yielded low mean seafood aroma liking scores for BCP (4.04) and GCP (4.13) at 2.66% HSP. The emotion “disgusted” was most affected by HSP addition for GCP. Presenting consumers with health benefit information (HBI) increased positive PI from 62.5% to 81.25% for BCP at 1.78% HSP, which had higher mean overall liking scores (6.05 before HBI, 6.24 after HBI) than 2.66% HSP samples. Overall liking was a significant predictor for positive PI with odds ratios of 1.52 to 2.20. Overall, green color and HSP addition levels of the coating inserted negative effects on liking, emotion, and PI of coated peanuts. This study demonstrated that HSP made from byproduct of squid processing could be successfully incorporated into coated peanuts, supporting the concept of sustainability of food supply. Practical Application In this study, the functional peptide ingredient made from squid head, a byproduct from seafood industry, was incorporated into coated peanuts. Effects of different coating colors (green compared with brown) and varying levels of hydrolyzed squid peptide powder on consumers’ seafood flavor perception, liking, emotions, and purchase intent of seasoned coated peanuts were clearly observed. The color of the coating material played an important visual cue that influenced product liking. Presenting consumers with health benefit information increased overall liking and positive purchase intent of the samples. Multisensory interactions of color and seafood aroma/flavor in these products should be further optimized.

Summary The novel peptides were successfully produced with commercial protease hydrolysis (Alcalase® and Flavourzyme®) at the fixed concentration of 3%by weight of protein in substrate. The hydrolysis was performed at various hydrolysis times (0, 300, 450, 600 and 750 min) at the optimal pH and temperature. When the hydrolysis was performed until 750 min, the peptide solutions with high soluble protein content were resulted (47.58 and 42.53 mg protein per mL for Alcalase® and Flavourzyme® hydrolysis, respectively). After freeze drying, the peptide powder from Alcalase® and Flavourzyme® hydrolysis had degree of hydrolysis at 71.87% and 66.39%, respectively. Alcalase® hydrolysis provided the novel peptide fraction with DPPH scavenging, ABTS activity and ACE‐inhibitory activity at 64.20%, 102.50 µg mL−1 and 56.98%, respectively. This peptide fraction possessed the lowest molecular weight (m/z 807.418) as detected by MALDI‐TOF/MS, and the most potent peptide sequence was Arg‐Glu‐Gly‐Tyr‐Phe‐Lys which has the potential as functional ingredient in novel food, snacks and beverages.

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