The ability of bacteria and archaea to modulate metabolic process, defensive response, and pathogenic capabilities depend on their repertoire of genes and capacity to regulate the expression of them. Transcription factors (TFs) have fundamental roles in controlling these processes. TFs are proteins dedicated to favor and/or impede the activity of the RNA polymerase. In prokaryotes these proteins have been grouped into families that can be found in most of the different taxonomic divisions. In this work, the association between the expansion patterns of 111 protein regulatory families was systematically evaluated in 1351 non-redundant prokaryotic genomes. This analysis provides insights into the functional and evolutionary constraints imposed on different classes of regulatory factors in bacterial and archaeal organisms. Based on their distribution, we found a relationship between the contents of some TF families and genome size. For example, nine TF families that represent 43.7% of the complete collection of TFs are closely associated with genome size; i.e., in large genomes, members of these families are also abundant, but when a genome is small, such TF family sizes are decreased. In contrast, almost 102 families (56.3% of the collection) do not exhibit or show only a low correlation with the genome size, suggesting that a large proportion of duplication or gene loss events occur independently of the genome size and that various yet-unexplored questions about the evolution of these TF families remain. In addition, we identified a group of families that have a similar distribution pattern across Bacteria and Archaea, suggesting common functional and probable coevolution processes, and a group of families universally distributed among all the genomes. Finally, a specific association between the TF families and their additional domains was identified, suggesting that the families sense specific signals or make specific protein-protein contacts to achieve the regulatory roles.

Sustainable development is an integrated blend of energetic, economic, social, environmental, and governmental actors, making it one of the most challenging and subjective aims of the United Nations. A sustainability project that does not consider any of these parameters can generate resistance among different actors of society. In this work, we have demonstrated that the traditional definition of sustainability encompassing economic development, environmental protection, and social justice does not adequately cover large-scale renewable energy projects. We have presented an illuminating case study of solar development in the Yucatan state of Mexico to show that the traditional three-legged stool of sustainable development fails to accommodate local power relations and their role in energy decision-making. This point is made through a substantive and illuminating ethnography of a solar project in the community of San José Tipceh, which involves the importance of energy democracy and understanding power relations in any assessment of sustainable development. These power relations are an important component of sustainable development, and all megaprojects, especially renewable energy projects, require special attention in the governance processes. Through an analysis of an example of implementation of a mega solar project, we argue that the methodology through which the power is exercised is an exercise of great importance because it leads us to a scientific discussion that structures an international reflection on sustainability. The case study consists of mixed methods, including several techniques to deepen the analysis of sociocultural aspects, and tries to present the limits and show the weakness of the sustainability perspective in such megaprojects. It is concluded that environmental justice as a basic element of the new era of renewable energies is limited and can generate social injustices. In the same way, this work shows how power is exercised in the implementation of energy projects and in the absence of equitable comprehensive governance that does not consider the local sociocultural contexts of the communities, resulting in the generation of new powers and permanent conflicts. At the end of this work, a framework to empower local sociocultural contexts for an equitable energy transition is recommended.

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