Analysis on economically viable indigenous food cereals like pearl millet as alternative strategies to curb under nutrition and boost food security is of utmost importance to widen the essential nutrient sources for human beings. To contribute to this area, macro and micro nutrient analysis was carried out on 60 pearl millet genotypes. On each of the genotype, 7 biochemical parameters (starch, amylose, amylopectin, protein, K, Zn and P) were analyzed. Starch content of the genotypes ranged from 27 -46.7% with a mean of 34.2%, while most of the genotypes had more amylopectin than amylose with exceptions of a few varieties with a ratio of 2:1. The protein content had a range of 4.6 -9.9% with a mean of 7.1%. Zinc was among the highest level followed by phosphorous and finally potassium. The principal component analysis (PCA) showed that the first four PCA contributed to 79.8% of the variability among the pearl millet varieties. Cluster analysis grouped data into 6 clusters and a singleton with a genetic distance 0.37 -8.73 showing great variability. Biochemical traits are useful tool for determining genetic variability in pearl millet and can contribute to breeding programs and enhance food security.