The dielectric characteristics of a material can be used as a non-destructive technique to evaluate and monitor the quality as well as the understanding of the structure-property of a material, through its dielectric properties as a function of frequency, temperature, chemical composition of the material, among others. In the literature there are few studies and data of dielectric characterization of films based on biopolymers. In this context, the objective of this research was the development and construction of an alternative instrumentation equipment on the market, such as network and impedance analyzers, which could be used for the dielectric characterization of biodegradable films based on gelatin. The method of parallel plates was used to determine the real part of permittivity known as relative permittivity or dielectric constant (ε'). The circuit used for the instrumentation was an astable oscillator operation based on operational amplifier (741) switched by the load of a parallel plate capacitor whose dielectric was a sample of the biodegradable film. From the values of the oscillation frequency and geometry of the capacitor, it was possible to calculate the capacitance of each sample and thus obtaining values of the relative permittivity of the film, using well established basic relationships. Gelatin films were produced by casting technique being used as plasticizer glycerol (G), sorbitol (S) and mixtures thereof, in proportion (G:S) 30:70, 50:50 and 70:30. The films were characterized for moisture and crystallinity. The relative permittivity (ε') of the films, determined at room temperature, was evaluated as a function of frequency (5-50 kHz), storage time, moisture content and type of plasticizer. The designed and constructed instrumentation was able to accurately measure the relative permittivity of the samples, being that this property decreased with increasing frequency for all films. Keeping constant frequency, there was no variation in ε' for the gelatin films, independent of the plasticizer over one month of storage at 24 ± 3 ⁰C. The moisture effect was observed at frequencies lower than 25 kHz, how bigger the moisture content the higher the relative permittivity. The effect of the plasticizer type in relative permittivity of the films were observed at low frequency (5 kHz) and plasticized films with sorbitol have higher ε' values. The plasticized films with higher moisture content exhibit lower crystallinity, hence larger molecular mobility and consequently higher the relative permittivity.