O objetivo deste trabalho foi quantificar a recuperação de larvas infectantes de Haemonchus sp. em capim Marandu (Brachiaria brizantha), no período da seca (inverno) e das chuvas (verão) ao longo do tempo; bem como verificar se a altura do pasto influenciou neste tempo de sobrevivência. O experimento foi conduzido em Uberlândia, durante os meses de agosto a novembro de 2014 (seca) e janeiro a março de 2015 (chuvas). Foram considerados dois tratamentos (alturas de pasto 10 e 35cm), com coleta de amostras nos dias 0, 7, 14, 28, 42, 56 e 70 dias. Houve contaminação experimental de parcelas com fezes de caprinos com média de 1600 ovos de estrongilídeos por grama (OPG). Foi realizada amostragem por cortes dos estratos inferior e superior do capim. Houve maior número de larvas recuperadas no estrato inferior no inverno (p<0,05). Não houve diferença no número de larvas recuperadas dos diferentes estratos no verão, nem entre as diferentes alturas de pasto (p>0,05). Foram encontradas larvas até o 42o dia no período chuvoso e até o fim do trabalho durante a seca, com maior quantidade de larvas (p<0,05) no 7º e 14º dia pós-contaminação. Pode se inferir a necessidade de estudos para maior conhecimento da influência das variáveis microclimáticas do pasto nas larvas infectantes de nematódeos.
Gastrointestinal parasites are a significant challenge in sheep farming, and periparturient ewes are one of the most susceptible categories in sheep flocks. This study aimed to verify whether the injection of B vitamins at the acupuncture point governing vessel 14 (GV14) can influence the parasitic load or host parasite interaction in naturally infected ewes during the peripartum period. Four weeks before parturition, 25 ewes were divided into five treatment groups: (1) Dose Control: 0.2 mL of B complex via IM; (2) Drug Control: 2 mL Complex B via IM; (3) Acupoint Control: 0.2 mL of Water at GV14 point; (4) Control; (5) Test group: 0.2 mL of B complex at the GV14 point. Treatments were performed at weeks -3, -1, 2, 4, and 6 about the parturition time. Fecal samples to estimate the parasitic load by fecal egg counts (FEC) and total blood samples for hemogram examination were performed seven days after the treatment, coinciding with weeks -2, 3, 5, and 7 about the parturition time. The number of peripheral eosinophils was higher in group 3 than in group 1 (p <0.05). Although groups 3 and 5 had lower mean values of FEC and higher hematological values at the time of weaning, these differences were not statistically significant (p> 0.05) according to the F test. However, all animals in groups 3 and 5 maintained low parasitism levels until the end of the experiment. Further studies should be performed with larger sample sizes and minor changes to the experimental protocol to elucidate the role of GV14 acupoint stimulation in the host-parasite relationship.
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