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Commonly, serological immunoassays and diagnostic kits include reference standard reagents (calibrators) that contain specific antibodies to be measured, which are used for the quantification of unknown antibodies present in the sample. However, in some cases, such as the diagnosis of allergies or autoimmune diseases, it is often difficult to have sufficient quantities of these reference standards, and there are limitations to their lot-to-lot reproducibility and standardization over time. To overcome this difficulty, this study introduces the use of surrogate recombinant calibrators formulated on the basis of two single-domain antibodies (nanobodies) combined through a short peptide linker to produce a recombinant bispecific construct. One of the nanobodies binds to the cognate analyte of the target antibody and the second is specific for the paratope of the secondary detecting antibody. The bispecific nanobody inherits the outstanding properties of stability and low-cost production by bacterial fermentation of the parent nanobodies, and once calibrated against the biological reference standard, it can be reproduced indefinitely from its sequence in a highly standardized manner. As a proof of concept, we present the generation and characterization of two bispecific calibrators with potential application for the diagnosis of allergy against the antibiotics aztreonam and amoxicillin in humans.

O presente ensaio visual faz parte de uma pesquisa monográfica (AUTORA, 2022, no prelo). Sua concepção ocorreu a partir das reverberações da escrita da pesquisa a/r/tográfica, em perspectiva autobiográfica, tendo tem como objetivo: elaborar um ensaio visual por meio de um processo poético-performativo enquanto reverberação de escritas no/do/pelo corpo. Aqui, retoma-se o que foi dançado-escrito ao longo da pesquisa.