The grains and sprouts of colored wheat genotypes (having blue, purple and yellow colored grains) contain specific anthocyanidins, such as pelargonidin and cyanidin derivatives, that produce beneficial health effects. The objective of the presented study is to compare the antioxidant capacity and contents of bioactive phytochemicals in grains and sprouts of wheat genotypes that differ in grain color. The methods α, α-diphenyl-β-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging activities, together with spectrophotometrical and high-performance thin-layer chromatography (HPTLC) methods, were used to study the presence of total phenolics, flavonoids, anthocyanins and anthocyanidins (pelargonidin, peonidin, cyanidin, delphinidin) content. It was predicted that the sprouts of all colored wheat genotypes would have significantly higher total flavonoids, total phenolics, anthocyanidin levels and antioxidant activity than the grains. The correlation results between antioxidant activity and contents of bioactive phytochemicals in grains and sprouts of colored wheat genotypes have shown a high correlation for cyanidin and pelargonidin, especially in grains, as well as quercetin in sprouts. It was found that total anthocyanin, quercetin and pelargonidin contents were significantly higher in the sprouts of the purple wheat genotypes than in the blue or yellow wheat genotypes. Delphinidin was detected at a higher level in the grains than in the sprouts of the blue wheat genotypes. Peonidin was present at very low quantities in the grains of all colored wheat genotypes. The sprouts of the purple wheat genotypes, among the colored wheat genotypes, had the highest pelargonidin, cyanidin and quercetin contents and, therefore, can be a promising source for functional food use.
The increase of spelt crop area is a result of overproduction of the basic cereals and the introduction of environmentally-friendly cultivation methods. Due to high interest in healthy lifestyle, healthy food and intensive development of organic agriculture, it was necessary to thoroughly examine the newly-formed cereal strains in order to register the best ones as varieties in the future. The experimental factors included: two weed control methods (mechanical and chemical), two sizes of sowing rate (300 and 500 seeds m-2) and the strains of spelt: three new breeding strains (STH 28-4609, STH 28-4614, STH 28-4619) and Oberkulmer Rotkorn cv. The research did not show protein diversity depending on a genotype, mean protein content reached 13% in grain dry mass. The plants weeded mechanically showed significantly highest crude fat content (2% DM). Mean value of crude fibre for all plants equalled 3.3% DM. The increase of neutral detergent fibre, acid detergent fibre and acid detergent lignin fractions was directly proportional to the increase in crude fibre content, which is beneficial for civilisation diseases prophylaxis. The studied agronomic factors did not significantly influence the essential amino acids content or total amino acids. The harrowed plants had grain with significantly increased nutrient content (crude protein, ether extract, crude fibre, ADF, cellulose, sulphur amino acids, leucine, and sum of essential amino acids) which is very important for organic farming.
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