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Opaque2 (O2) is a bZIP transcriptional regulatory factor involved in the control of seed storage proteins synthesis as well as carbon and nitrogen metabolism during maize seed development. Phylogenetic analysis of a possible complete and nonredundant collection of angiosperm bZIP factors resulted in the identification of 20 angiosperm O2-homologues that defined what we call the O2 gene family. Members of the family share a highly conserved bZIP DNA binding domain and several other motifs which define important functional features. The O2 family was enriched by the identification of 25 new putative angiosperm O2 homologous genes in EST databases and in the rice genome. Based on parsimony analysis, the collection of O2 homologues was organized into one eudicot-monocot and three monocot groups of orthologous genes and two groups of eudicot genes. These results support a model of the evolution of the O2 family that involves two O2 homologous gene duplications before the separation of monocots and eudicots. Further expansion of O2 homologues resulted in at least three and one gene duplications in the monocot and eudicot lineages, respectively. O2 appears to have been the result of a monocot-specific gene duplication event, and the possibility that O2 represents a functional specialization restricted to monocots is suggested.

Sugarcane is generally propagated by cuttings of the stalk containing one or more lateral buds, which will develop into a new plant. The transition from the dormant into the active stage constitutes a complex phenomenon characterized by changes in accumulation of phytohormones and several other physiological aspects. Abscisic acid (ABA) and methyl-jasmonate (MeJA) are major signaling molecules, which influence plant development and stress responses. These plant regulators modulate gene expression with the participation of many transcriptional factors. Basic leucine zipper proteins (bZIPs) form a large family of transcriptional factors involved in a variety of plant physiological processes, such as development and responses to stress. Query sequences consisting of full-length protein sequence of each of the Arabidopsis bZIP families were utilized to screen the sugarcane EST database (SUCEST) and 86 sugarcane assembled sequences (SAS) coding for bZIPs were identified. cDNA arrays and RNA-gel blots were used to study the expression of these sugarcane bZIP genes during early plantlet development and in response to ABA and MeJA. Six bZIP genes were found to be differentially expressed during development. ABA and MeJA modulated the expression of eight sugarcane bZIP genes. Our findings provide novel insights into the expression of this large protein family of transcriptional factors in sugarcane.