The objective of the present study was to prepare a Dihydroartemisinin microparticles using spray drying method to enhance the solubility and dissolution rate which will help for improvement of oral bioavailability. Dihydroartemisinin is the active metabolite of artemisinin compound and a drug of choice for treatment of malaria. Dihydroartemisinin has poor aqueous solubility, incompletely absorbed after oral intake due to poor dissolution characteristics in the intestinal fluid which limits its application. To address these issues, spray dried microparticles of Dihydroartemisinin were prepared using Poloxamer 188, non-ionic surfactant with different ratios of drug and carrier. Based on results of solubility study, spray dried microparticles formulation with highest aqueous solubility was analyzed for Fourier transform infrared spectroscopy, X-ray powder diffraction and Differential scanning calorimetry study. Spray dried microparticles evaluated for determination of percent yield, solubility, drug content, encapsulation efficiency, micromeritic properties and in vitro dissolution parameters. Surface morphology study by Scanning electron microscopy revealed spherical shape of microparticles. Prepared microparticles showed good flow property, improved aqueous solubility and faster in vitro dissolution rate compared with pure drug. The spray dried microparticles of Dihydroartemisinin with Poloxamer 188 in a ratio of 1:4 w/w showed 2.5 times enhancement of aqueous solubility and > 95 % drug release in 120 min when compared with pure drug alone. Thus from the result it can be concluded that spray dried microparticles of Dihydroartemisinin using Poloxamer 188 is useful technique to enhance the solubility and dissolution rate that will help to improve oral bioavailability.