Introduction: Epidermolysis bullosa (EB) is a rare genetic skin disorder inherited either in autosomal recessive (AR) or autosomal dominant (AD) manner and characterized by blistering of the skin and mucous membranes. According to a subtype of EB, the oral manifestations and dental involvement vary in frequency and in severity. The most severe dental problems occur in patients with junctional epidermolysis bullosa (JEB) and severe generalized dystrophic epidermolysis bullosa (RDEB) and involve enamel erosion and development of blisters followed by painful oral wounds. Oral mucosa lesions decrease patients' quality of life and may contribute to difficulties in nutrition leading to cachexia. Aim: Assessment of efficacy of gentamicin 0.3% solution in the healing and preventing of oral erosions in patients with RDEB and evaluating its impact on the expression of type VII collagen. Material and methods: The study included four female patients with RDEB, aged 16-42 who show different mutations in the COL7A1 gene and were administered the mouth rinse two times daily with a solution of 0.3% gentamycin for 4 consecutive weeks. Prior to and at the end of the study, the samples from oral mucosa were collected to estimate the expression of type VII collagen by immunofluorescence test. Results: The clinical improvement of oral wounds healing and reduced number of new blisters and mucous membrane soreness as well as partial re-expression of type VII collagen was observed in all studied patients. Conclusions: Topical gentamicin therapy of oral cavity in RDEB patients resulted in clinical improvement of mucosal lesions and re-expression of collagen type VII.

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Bisphosphonates are a group of medicines used in the treatment of oncological osteoporosis, Paget disease and osteogenesis imperfecta. They significantly interfere with the regeneration processes of bone tissue and have a tendency to accumulate in the areas of increased bone remodeling, i.e., the maxilla and the mandible. One of their most serious and problematic side effects is the induction of bisphosphonaterelated osteonecrosis of the jaw (BRONJ), characterized by an exposed, necrotic bone in the maxillofacial area, which lasts longer than 8 weeks, in the case of a patient who is using or was using bisphosphonates and who did not undergo radiotherapy in this anatomical area. Dentistry manipulations are one of the factors which may increase the risk of BRONJ occurrence. The existing recommendations for preventing osteonecrosis are ambiguous. Some authors recommend that the bisphosphonate therapy be discontinued prior to dental procedures, while others say that there is no evidence for the effectiveness of interrupting the therapy. There is also no unequivocal attitude toward antibiotic prophylaxis. According to this research, each case should be considered individually, primarily having regard to the good of a patient.

Background : Hypodontia causes significant developmental disorders of the bones of the jaws. In its course, the development of the affected part of the alveolar ridge is inhibited. In the case of oligodontia or anodontia, gnatic malocclusions and defects may develop, accompanied by disturbances of speech, swallowing and even breathing. Treatment of malformation of the jaws due to lack of tooth buds is long-term and does not often give satisfactory results. Orthodontic and surgical treatment are used.Aim/Hypothesis : The aim of the study was to assess, on an animal model of a pig, the possibility of using selected growth factors used in a surgical clinic to limit the development of morphological disorders of the mandible occurring in the course of anodontia. Material and Methods :The research was carried out on the basis of the consent of the ethics committee issued for the purposes of the grant of the National Science Center. The study was conducted on 10 pigs at the age of 7 days. In inhaled general anesthesia, unilateral germectomy of molar teeth in the mandible was performed. Four test groups were distinguished-A. germectomy without augmentation B. germectomy with β-TCP augmentation C. germectomy with PDGF augmentation on β-TCP carrier D. germectomy with rh-BMP-2 augmentation on a collagen carrier. The control group was left without surgery. After 3 months, the animals were sacrificed with pentobarbital 100 mg kg. Mandibles of

Background : Primary stability of dental implants depends on implant length and diameter, its structure, quality and quantity of the bone and technique of bone-bed preparation. Implants` cradles can be prepared using cylindrical drills (conventional preparation) or piezosurgery instruments. Previous studies of other authors have reported data assessing effectiveness of the technique of bone-bed preparation on primary implant stability, but they are ambiguous.Aim/Hypothesis : The aim of the study is to compare the impact of different bone-bed preparation techniques such as conventional drilling and piezosurgery on primary implant stability.Material and Methods : AB dental cylindrical implants of different diameter (3.75, 4.2, 6 mm) and length (8, 11.5, 16 mm) were used. The implants` cradles were prepared using conventional preparation by cylindrical drills and piezosurgery technique. The implants were inserted into isolated pork shoulders. Before implants' installation, all of them were marked by gutta-percha points and scanned with computed tomography for compact bone thickness and bone density measurements. Together 54 implants' cradles were performed -three cradles for the each implant in each of the group (conventional and piezosurgery technique). Bone-beds were prepared according to the manufacturer`s recommendations. Next, a torque by a dynamometric key, a primary stabilization with Periotest and Osstell Mentor were performed. All measurements were averaged and statistically analyzed using ANOVA procedure of SAS System 9.2.Results : High values of primary stability were obtained for conventional and piezosurgery technique. On the basis of the results achieved during the study the following relationships have been identified-ï The mean value of insertion torque is higher for piezosurgery technique (60 ± 0) than for conventional one (58.33 ± 4.80) in D2 bone.ï The mean value of insertion torque is higher for piezosurgery technique (58.89 ± 3.2) than for conventional one (55.56 ± 6.98) in D3 bone.ï There is no statistically significant differences between Periotest and Osstell Mentor measurements for both bone-bed preparation techniques. Conclusion and Clinical Implications: On the basis of the results achieved during the study, it is not possible to state clearly which of bone-bed preparation technique is more effective in achieving high primary stability. In each clinical case, the method of preparation should be individually determined according to the patient ' s and the operator ' s preferences.