The aim of the experiment was to determine the effects of synthetic auxin and cytokinin and the time of their application on some morphological and physiological characteristics of Medicago x varia T. Martyn grown under controlled conditions. The experiment was to check whether an application of exogenous hormones during vegetative and generative stages of the plant had an effect on above-ground mass development, on nitrate reductase activity and on plastid pigments content. Experiment factor was synthetic auxin and cytokinin and the date of their application. Auxin was applied in the form of a synthetic indole-3-butyric acid, while cytokinin was sprayed as synthetic 6-benzylaminopurine. The control plants were treated with distilled water. Depending on the experimental variant, spraying was applied at the sixth true leaf stage and at the first flower bud stage. The research showed that the response of the alfalfa plants to the application of cytokinin and auxin was not uniform. It seems that the most effective was the application of a mixture of them both but only during the vegetative stage.Additionally, cytokinin caused an increase in plastid pigments content in alfalfa leaves. On the other hand, a mixture of auxin and cytokinin triggered the highest nitrate reductase activity in alfalfa roots and raised the ratio of total chlorophyll content to carotenoids. Synthetic auxin caused the decrease of the levels of most parameters compared to the control.

The aim of the research was to evaluate the effect of foliar application of different doses of Tytanit as a biostimulant on the yield and nutritional value of Medicago × varia T. Martin and Trifolium pratense L. It was assumed that titanium application during any life cycle of alfalfa hybrid and red clover would contribute to their growth, digestibility, and total protein content. In addition, it was expected that increasing doses of Tytanit up to 0.6 dm 3 ha −1 would improve the yield and quality of forage plants. Different doses of Tytanit in different ways affected the yield. However, the largest dose of 0.6 dm 3 turned out to be the most effective. It contributed to a 38% increase in the yield of hybrid alfalfa and to a 31% increase in the red clover yield. Individual doses of Tytanit in different ways affected accumulation of protein and crude fibre in the dry matter. Used at 0.4 and 0.6 dm 3 doses it increased the amounts of protein and crude fibre relative to control. The smallest dose had no significant effect on these parameters. Tytanit did not improve dry matter digestibility, and there was no statistically significant variation as a result of its application. Foliar application of the biostimulant resulted in an increase in the concentration of phosphorus, potassium, and magnesium in plant dry matter. High content of calcium in the plant species before Tytanit application increased further as a response to 0.2 and 0.4 dm 3 doses, with a slight increase in the ratio of K: (Ca + Mg) and an excessive growth of the Ca: P ratio, which reduced hybrid alfalfa and red clover nutritional values. Thus, Tytanit doses used in the experiment significantly increased hybrid alfalfa and red clover yields, but the nutritional value of the plants did not improve.