SummaryParenchyma cells from the inner mesocarp of a grape berry (Vitis vinifera L. cv. Chardonnay) were visualised in three-dimensions within a whole mount of cleared, stained tissue using confocal laser scanning microscopy and digital image reconstruction. The whole berry was ®xed, bisected longitudinally, cleared in methyl salicylate, stained with safranin O and mounted in methyl salicylate. Optical slices were collected at 1.0 mm intervals to a depth of 150 mm. Neighbouring z-series were joined post-collection to double the ®eld-of-view. Attenuation at depth of the¯uorescent signal from cell walls was quanti®ed and corrected. Axial distortion due to refractive index mismatch between the immersion and mounting media was calibrated using yellow-green uorescent microspheres and corrected. Transmission electron microscopy was used to correct¯uorescent measurements of cell wall thickness. Digital image reconstructions of wall-enclosed spaces enabled cells to be rendered as geometric solids of measurable surface area and volume. Cell volumes within the inner mesocarp tissue of a single grape berry exhibited a 14-fold range, with polysigmoidal distribution and groupings around speci®c size classes. Cell shape was irregular and the planes of contact were rarely¯at or simple. Variability in cell shape was indicated by the range in surface area to volume ratios, from 0.080 to 0.198 mm ±1 . Structural detail at the internal surface of the cell wall was apparent. The technique is applicable to a wide range of morphometric analyses in plant cell biology, particularly developmental studies, and reveals details of cell size and shape that were previously unattainable.