Introduction: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. Methods: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis. Results: The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis. Conclusions: The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.
A Dietilnitrosamina (DEN), uma substância reconhecidamente hepatotóxica e carcinogênica, foi utilizada na indução da necrose hepática centrolobular em ratos isogênicos Lewis divididos em 5 grupos de 5 animais. O objetivo deste estudo foi avaliar o efeito quimiopreventivo da epigalocatequina-3-galato (EGCG), de Camellia sinensis (chá verde) no tratamento da hepatotoxicidade celular induzida pela DEN. Foi mensurada a concentração sérica da alanina aminotransferase (ALT) e aspartato aminotransferase (AST) dos diferentes grupos experimentais. No ensaio bioquímico para AST e ALT, houve diferença
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