Background: A systematic training program has not been developed for pulmonary artery catheter (PAC) patient care in Taiwan; only the mentor-apprentice learning approach is used. Therefore, it is difficult for nursing personnel to maintain mastery of care. Methods: ICU nurse practitioners of regional teaching hospitals were enrolled in this study to perform pretest, post-test, and repeated measurements in order to examine the long-term learning effectiveness of this intervention. Before and after e-learning using the PAC Teaching DVD and high-tech simulation interventions, this study used scales to assess knowledge, attitude, technique inspection, and satisfaction with the PAC Teaching DVD to collect data. Results: The differences in the posttest scores and repeated measurements in the areas of knowledge and techniques of PAC patient care reached significance (p < .05). The differences in scores in the care attitude areas of "Beneficial to the improvement of self-confidence in care" and "I believe that I can provide accurate data for decision-making" post-test and those in the area of "During insertion of a pulmonary artery catheter in patients, I will look up information to look for answers" reached significance (p < .05). The learners were all satisfied with the PAC Teaching DVD. Conclusion: This study considered the needs of learners, and developed the PAC Teaching DVD according to theory. The results showed that the interventions had a long-term learning effectiveness in terms of knowledge, attitude, and techniques of PAC patient care. This research can be used as a reference for training in the care of PAC patients.