Reactive
oxygen species (ROS) are an important component for maintaining
normal physiological activities in organisms, and abnormal changes
in their level are often accompanied by many diseases. As the two
most representative components of ROS, HClO and H2O2 play vital roles in many physiological and pathological processes
and are interdependent and mutually transformable. Although there
is a lot of work that has specifically detected HClO or H2O2, there are few reports on the simultaneous differential
detection of HClO and H2O2. Here, we report
a ratio-based fluorescent probe capable of simultaneously distinguishing
HClO and H2O2 based on making the best use of
the untapped potential of coumarin derivatives. This probe was triumphantly
put into use in the discriminative identification of HClO and H2O2 in aqueous media with high sensitivity and selectivity,
and the probe was appropriate in a wide pH range. Furthermore, the
imaging experiment for HClO and H2O2 in cells
and zebrafish was eventually proven to be feasible. Importantly, this
probe was qualified for monitoring the variation of HClO and H2O2 levels in organisms with alcoholic liver injury.
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