Background: Intervertebral disc degeneration (IDD) is a heterogeneous spinal disease, and its underlying molecular mechanism is unclear. Objectives: This study was aimed to identify, profile, and analyze microRNAs (miRNAs) that are related to IDD. Method: Microarray gene expression IDD data (GSE63492) were downloaded from Gene Expression Omnibus datasets. We employed weighted gene co-expression network analysis (WGCNA) to construct a miRNA co-expression network and the miRNAs related to IDD stage were detected. The number of differentially expressed miRNAs between normal and degenerated nucleus pulposus tissues was calculated. Twenty-three clinical specimens were used to validate the expression of miRNAs using qRT-PCR. Results: WGCNA identified 48 miRNAs that were significantly related to IDD stage, and 94 miRNAs were significantly different between normal and degenerated nucleus pulposus tissues. We selected 32 overlapping miRNAs and identified 347 corresponding target genes. The integrative analysis revealed the biological function and pathways of these targeted genes. Analysis of clinical specimens validated that hsa-miR-4534 was upregulated in IDD, whereas hsa-miR-1827 and hsa-miR-185-5p were downregulated in IDD.