Instantaneous self-assessment (ISA) is a technique that has been developed as a measure of workload to provide immediate subjective ratings of work demands during the performance of primary work tasks such as air traffic control. This paper reports a study that compared the results of ISA with those gathered from other established workload evaluation techniques; subjective ratings collected at the end of the task, mean heart rate and heart rate variability, and error in the primary task of tracking. ISA ratings were found to be correlated significantly with the post-task ratings of workload, heart rate variability, and task performance. Generally each of the techniques was sensitive to variations in task difficulty. However, performance on the primary tracking task was found to be poorer during periods when ISA responses were required, regardless of whether they were spoken or manual responses. This finding suggests that the usefulness of the technique is limited in comparison to less intrusive measures of workload.