Abstract:We developed a compact plane illumination plugin (PIP) device which enabled plane illumination and light sheet fluorescence imaging on a conventional inverted microscope. The PIP device allowed the integration of microscope with tunable laser sheet profile, fast image acquisition, and 3-D scanning. The device is both compact, measuring approximately 15 by 5 by 5 cm, and cost-effective, since we employed consumer electronics and an inexpensive device molding method. We demonstrated that PIP provided significant contrast and resolution enhancement to conventional microscopy through imaging different multi-cellular fluorescent structures, including 3-D branched cells in vitro and live zebrafish embryos. Imaging with the integration of PIP greatly reduced out-of-focus contamination and generated sharper contrast in acquired 2-D plane images when compared with the stand-alone inverted microscope. As a result, the dynamic fluid domain of the beating zebrafish heart was clearly segmented and the functional monitoring of the heart was achieved. Furthermore, the enhanced axial resolution established by thin plane illumination of PIP enabled the 3-D reconstruction of the branched cellular structures, which leads to the improvement on the functionality of the wide field microscopy. 1938-1940 (2007

A compact Ka-band (26.5-40 GHz) dielectric rod end-fired antenna array for the three-dimensional (3D) active holographic imaging system is presented. Without horn shape feeding, the new antenna array is specially designed with characteristics such as small size, high gain, good radiation pattern, easy realisation, low insertion loss and low mutual coupling. In the imaging system, the spacing of sending and receiving antenna units is 1.5 wavelengths with mutual coupling <−30 dB and a gain of 12-16 dB. The results and analysis provide guidelines for the design of millimetre-wave end-fired antenna arrays.

No abstract

No abstract