This study aimed to evaluate performance and behaviour of Nellore steers (Bos taurus indicus) in integrated systems. Treatments consisted of three different systems: livestock; crop-livestock; and livestock-forestry, with triple rows of eucalyptus trees. The experiment was carried out in Sinop, MT, Brazil, in a randomised complete block design with four replicates, during two seasons (dry-rainy transition and rainy). The experimental period was from 19 September 2015 to 10 January 2016. Nellore steers used in the experiment had an average initial weight of 344 kg ± 11 kg, in continuous stocking and variable stocking rate. Forage mass accumulation rate was evaluated and, independently of the system, had the highest values recorded during the rainy season. Microclimate was evaluated through temperature and humidity index, black globe temperature and humidity index and radiant heat load. Highest values were obtained from 11:00 a.m. to 3:00 p.m. at the dry-rainy transition period for all systems. Animal behaviour was assessed every 10 min from 7:00 a.m. to 5:00 p.m., and averages were calculated for every 2 h. The longest grazing period was recorded from 9:00 a.m. to 1:00 p.m., and then after 3:00 p.m., regardless of the system. Rumination occurred more frequently from 1:00 p.m. to 3:00 p.m. during the rainy season, and also in the shade for the livestock-forestry system. Highest weight gains per area were recorded during the rainy season and for the crop-livestock system. It is concluded that the highest yields can be achieved with the inclusion of the crop component in livestock production systems, and that Nellore steers in silvopastoral systems prefer to ruminate and stay idle in the shade.

To meet the global demand for animal protein, sustainable intensification of existing livestock systems may be possible, especially through integration of livestock with crops or forestry. Thus, our objective was to compare forage production and animal performance in grass monoculture and integrated systems in the Brazilian Amazon biome. The four systems were (a) livestock (L) with Marandu palisadegrass {Brachiaria brizantha (Hochst. ex A. Rich.) R. D. Webster} as monoculture, (b) palisadegrass pastures integrated with eucalyptus trees (Eucalyptus urograndis; hybrid of Eucalyptus grandis W. Hill ex Maiden and Eucalyptus urophylla S. T. Blake) arranged in three‐row groves with groves spaced 30 m apart (livestock–forestry; LF), (c) palisadegrass after two years of crops (crop–livestock; CL) and (d) palisadegrass after two years of crops with single rows of eucalyptus trees spaced 37 m apart (crop–livestock–forestry; CLF). From July 2016 to July 2017, all experimental units were continuously stocked using a variable stocking rate. Greater herbage accumulation (HA) occurred in CL and CLF in comparison with L and LF (21,310, 24,050, 19,500 and 18,890 kg DM/ha respectively). The gain per hectare of L, LF and CL (average of 932 kg ha–1) was less than CLF (1,190 kg ha–1). Average daily gain was similar among systems (0.69 kg/day). We conclude that integrated systems can support similar (LF or CL) or greater (CLF) levels of animal production than palisadegrass monocultures while increasing diversity of outputs, thereby providing a greater range of viable systems for livestock production in the Brazilian Amazon biome.

The objectives of this study were: 1) to compare the effects of live yeast (LY), yeast fermentation product (YFP), a mix of Lactobacillus acidophilus and Propionibacterium freudenreichii (MLP), and Lactobacillus plantarum included as additives in dairy cows’ diets on in vitro ruminal fermentation and gas production (GP); and 2) to evaluate the effects of L. plantarum as direct-fed microbials (DFM) in dairy cows’ diets on in vitro ruminal fermentation, GP, nutrient digestibility, and N metabolism. Three experiments were carried out: Exp. 1 had the objective to compare all additives regarding ruminal fermentation parameters: an Ankom GP system was used in a completely randomized design, consisting of four 48 h incubations, and eight replications per treatment. There were eight treatments: a basal diet without additive (CTRL) or with one of the following additives: LY, YFP, MLP, or L. plantarum at four levels (% of diet Dry Matter (DM)): 0.05% (L1), 0.10% (L2), 0.15% (L3), and 0.20% (L4). In Exp. 2, a batch culture was used to evaluate ruminal fermentation, and CO2 and CH4 production using the same treatments and a similar experimental design, except for having 16 replications per treatment. Based on Exp. 1 and 2 results, Exp. 3 aimed at evaluating the effects of the L. plantarum on ruminal true nutrient digestibility and N utilization in order to evaluate the use of L. plantarum as DFM. The treatments CTRL, MLP, L1, and L2 were used in a replicated 4 × 4 Latin square design using a dual-flow continuous culture system. Data were analyzed using linear and nonlinear regression; treatment means were compared through contrasts, and L treatments in Exp. 1 and 2 were tested for linear, quadratic, and cubic effects. In Exp. 1, all treatments containing additives tended to reduce OM digestibility as well as reduced total volatile fatty acids (VFA) concentration and total GP. The YFP had greater OM digestibility than LY, and MLP treatment had greater total VFA concentration compared to L. plantarum treatments. In Exp. 2, additives reduced CO2 production, and there were no major differences in CH4. In Exp. 3, all additives reduced NH3-N concentration. In conclusion, pH and lactate concentration were not affected in all three experiments regardless of additive tested, suggesting that these additives may not improve ruminal fermentation by pH modulation; and L. plantarum may improve ruminal N metabolism when used as DFM in high-producing dairy cows’ diets, mainly by reducing NH3-N concentration.

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The objective of this experiment was to evaluate the effects of supplementing a rumen-protected source of Met, N-acetyl-l-methionine (NALM), on lactational performance and nitrogen metabolism in early-to midlactation dairy cows. Sixty multiparous Holstein dairy cows in early lactation (27 ± 4.3 d in milk, SD) were assigned to 4 treatments in a randomized complete block design. Cows were blocked by actual milk yield. Treatments were as follows: (1) no NALM (control);(2) 15 g/d of NALM (NALM15); (3) 30 g/d of NALM (NALM30); and (4) 45 g/d of NALM (NALM45). Diets were formulated using a Cornell Net Carbohydrate and Protein System (CNCPS) v.6.5 model software to meet or exceed nutritional requirements of lactating dairy cows producing 42 kg/d of milk and to undersupply metabolizable Met (control) or supply incremental amounts of NALM. The digestible Met (dMet) supply for control, NALM15, NALM30, and NALM45 were 54.7, 59.8, 64.7, and 72.2 g/d, respectively. The supply of dMet was 88, 94, 104, and 115% of dMet requirement for control, NALM15, NALM30, and NALM45, respectively. Milk yield data were collected, dry matter intake (DMI) was measured daily, and milk samples were collected twice per week for 22 wk. Blood, ruminal fluid, urine, and fecal samples were collected during the covariate period and during wk 4, 8, and 16. Data were analyzed using the GLIMMIX procedure of SAS (SAS Institute) using covariates in the model for all variables except body weight. Linear, quadratic, and cubic contrasts were also tested. Treatments did not affect DMI, milk yield, and milk component concentration and yield; however, feed efficiency expressed as milk yield per DMI and 3.5% fat-corrected milk per DMI were quadratically affected, with greater response observed for NALM15 and NALM30 compared with control. Acetate proportion linearly increased, whereas propionate proportion linearly decreased with NALM supplementation. Blood urea nitrogen linearly decreased with NALM supplementation. Total plasma essential AA concentrations were quadratically affected, as greater values were observed for control and NALM45 than other treatments. Plasma Met concentration was quadratically affected as lower levels were observed with NALM15, whereas Met concentrations increased with NALM45 compared with control. Nitrogen utilization efficiency and apparent total-tract nutrient digestibility were not affected by treatment. Supplementation of NALM at 15 or 30 g/head per day resulted in the greatest improvements in feed efficiency without affecting N metabolism of early-to mid-lactation dairy cows.