The FlowMetrixTM System is a multiplexed data acquisition and analysis platform for flow cytometric analysis of microsphere-based assays that performs simultaneous measurement of up to 64 different analytes. The system consists of 64 distinct sets of fluorescent microspheres and a standard benchtop flow cytometer interfaced with a personal computer containing a digital signal processing board and Windows95®-based software. Individual sets of microspheres can be modified with reactive components such as antigens, antibodies, or oligonucleotides, and then mixed to form a multiplexed assay set. The digital signal-processing hardware and Windows95-based software provide complete control of the flow cytometer and perform real-time data processing, allowing multiple independent reactions to be analyzed simultaneously. The system has been used to perform qualitative and quantitative immunoassays for multiple serum proteins in both capture and competitive inhibition assay formats. The system has also been used to perform DNA sequence analysis by multiplexed competitive hybridization with 16 different sequence-specific oligonucleotide probes.

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This document is xubject to special expcrt contaols and ecch transmittal to foreign governments or foreign nationals may be r,,e only with prior 9)Leroval of the Directorate of Airframe Subsystems Engineering (SEFL), Systems Engineering Group, Wrigt-Patterson AFl, Ohio. SYSTEMS ENGINEERING SEG-TR-( 7-19NBSTRACT Because of recent laigh rejection, rates on carbon steel control ables Mn military aircraft, the Air Force oonduotod breaking strength and epidurance tests on various types of aircraft oontrol oables. Since approximately 90% of the service life of jet aircraft -is flown at low temperatures, the primary purpose of the tests wss to obtain information on cable fatigue life at low temperature. The tests were conducted on 1/8-inch disx eter 7x1,9 galvanized carbon steel tin-coated c a rbon steel, aid stainless steel cables at both low temperature~ (-Gr*F) and room~ tempers~ure (+70F). The tests were conducted in the Climatic Laboratory at Eglin AFB, Florida, l~ebults indicate thatthe stainless steel cable.s are far' superior to the galvanized or tin-coated cables kcr low ternperature operation. Therefore, the stainless bteel cables should be considered for replacement of the carbon r34eel control cables on mititr aircraft, and the appropriate specifications should be updated accordling7y.(rhis abstract is subject to special excport controls and each transmittal to foreign governments or foreign nationals may be made only with prior approval of the Directorate of Airframe Subsysteras Engineering (SEFL), Systems Engineering Group, Wright-Patterson Air Force Base, Ohio.) jmI7 ?AMX WAS MAW, TWH9U1= UAS NOT rlXUD.