This paper reviews the developments in dielectric elastomer actuator technology for several applications. Dielectric elastomers are a variety of electroactive polymer that deform due to the electrostatic interaction between two electrodes with opposite electric charge. Dielectric elastomers have been subject of much interest and research over the past decade. In earlier years, much of the focus was on actuator configurations, and in more recent years the focus has turned to investigating material properties that may enhance actuator performance. This review outlines the operating principle and actuation mechanisms behind this actuator technology, highlights some of its advantages over existing actuator technologies, identifies some of the challenges associated with its development, and examines the main focus of research within this field, including some of the potential applications of such an actuator technology.
Mesenchymal stem cells (MSCs) within their native environment of the stem cell niche in bone receive biochemical stimuli from surrounding cells. These stimuli likely infl uence how MSCs differentiate to become bone precursors. The ability of MSCs to undergo osteogenic differentiation is well established in vitro; however, the role of the natural cues from bone's regulatory cells, osteocytes and osteoblasts in regulating the osteogenic differentiation of MSCs in vivo are unclear. In this study we delineate the role of biochemical signalling from osteocytes and osteoblasts, using conditioned media and co-culture experiments, to understand how they direct osteogenic differentiation of MSCs. Furthermore, the synergistic relationship between osteocytes and osteoblasts is examined by transwell co-culturing of MSCs with both simultaneously. Osteogenic differentiation of MSCs was quantified by monitoring alkaline phosphatase (ALP) activity, calcium deposition and cell number. Intracellular ALP was found to peak earlier and there was greater calcium deposition when MSCs were co-cultured with osteocytes rather than osteoblasts, suggesting that osteocytes are more infl uential than osteoblasts in stimulating osteogenesis in MSCs. Osteoblasts initially stimulated an increase in the number of MSCs, but ultimately regulated MSC differentiation down the same pathway. Our novel coculture system confi rmed a synergistic relationship between osteocytes and osteoblasts in producing biochemical signals to stimulate the osteogenic differentiation of MSCs. This study provides important insights into the mechanisms at work within the native stem cell niche to stimulate osteogenic differentiation and outlines a possible role for the use of co-culture or conditioned media methodologies for tissue engineering applications.
Customised properties of parts manufactured using the selective laser sintering process are achievable by variation of build parameters. The energy density, controlled by laser power, distance between scan lines and speed of the laser beam across the powder bed, all have a very strong influence on the density and the mechanical behaviour of the parts. The present paper investigates the influence of the energy density on physical and mechanical properties of parts produced using polyamide. Additionally, the effect of part orientation during the build is examined. Knowledge of the influence of these parameters allows one to establish trendlines which link build settings to resulting part properties, and hence to fabricate customised parts with predetermined properties.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.