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differences in DNA structure within tumor initiation and tumor promotion sites. Right-handed double-stranded (ds-) B-DNA is the conventional structure of DNA that results in the majority of DNA. As part of our past work we examined the epidermis of normal human skin for the presence of ds-B-DNA as it undergoes destruction due to normal cell death processes [i.e., apoptosis and terminal differentiation (denucleation)]. Our research team has examined the distribution and intensity of anti-B-DNA antibody binding in human melanoma; formalin-fixed paraffin-embedded tissue sections (1 micron). We also employed a variety of different anti-melanoma antibody probes [e.g., (HMB45) (ab787)]. Using enhanced histotechnological processing procedures we were able to better preserve the melanoma tissue-bound B-DNA (i.e., intact, unaltered and non-denatured nucleic acids). Superior preservation of tissuebound components resulted in improved characterization of the immunostaining data (1). We characterized the lateral and vertical margins of the epidermal tumor growth to see if any changes were occurring in the non-cancerous areas of the epidermis next to the tumor sites. We found that B-DNA is located in all cells, and that the binding intensity [mean optical density] of immunohistochemistry is similar in all regions of the cancerous growth. Being able to locate hyperactive regions of B-DNA in the tumor growth will allow for new drug target sites. disease. 1. Gagna C.E., et al., (2007