Purpose: The purpose of the study was to examine the extent to which training influences implementation of performance appraisal in public Universities in Mount Kenya Region. Methodology: This study adopted descriptive survey research design. The target population of this study was the staff, human resource officers, administrators and finance officers in the 7 chartered public universities as they are considered as the ones directly involved in the implementation of performance appraisal systems. The target population for this study consisted of 2423 respondents from which a sample size was drawn. The sample size was 136 respondents from the 7 chartered public universities in Mount Kenya Region. Stratified random sampling was used in this study. The study collected primary data by use of questionnaire. Quantitative data was analyzed by the use of descriptive statistics using SPSS (Version 20) and figures and tables were used to present the results. Both correlation and regression analyses were used to show the association between the variables. Findings: Regression results indicated that training had a positive and significant effect with implementation of performance appraisal systems. The study concluded that well designed training programs helps to improve the knowledge, skills and abilities of employees to achieve the organization’s strategic plan. Recommendations: It was recommended that universities should have training of their staff to ensure competence which will in the long run improve implementation of performance appraisal systems. In addition, training the staff will help the organization to augment the advantages acknowledged through the usage of venture, program, and portfolio administration programming. Moreover competent staff ensure faster implementation of projects as they know the part to play.