The aim of this study was to prove the hypothesis that the growth and health of calves are dependent on feed supplements with an antidiarrhoeic effect, in relation to sex, season of birth, and number of the dam's lactations. A total of 186 calves were included in the experiment. After birth the calves were divided into three treatment groups: Ascophyllum nodosum (brown seaweed hydrolyzate, prebiotics), Lactobacillus sporogenes (probiotics), and the control group. All calves were weighed within two h after birth. The growth and health were investigated from the birth to the fourth week of age. Compared to the control, a significant effect of applied feed supplements was found in the Lactobacillus sporogenes group in the body weight at 28 days of life (P < 0.01) and in the average daily gains (P < 0.001). Differences between sexes were found in the body weight at birth (P < 0.001) and in the body weight at 28 days of life (P < 0.01). The effect of the season of birth was recorded in the average daily gains (P < 0.01). The effect of the number of the dam's lactations on calf was proved in body weights at birth and 28 days of life (P < 0.01). The interaction between treatment and sex (P < 0.05), and between treatment and season of birth (P < 0.01) were calculated in the average daily gains. We concluded from the analysis that only the use of Lactobacillus sporogenes had a positive influence on increasing the growth. Neither of the two supplements had a positive impact on the health of calves.

The report describes a unique case of newborn conjoined twins in guinea pigs (Cavia aperea f. porcellus). Connected twins in guinea pigs have been very rare, and there are only a few previous reported cases. The newborns were stillborn in breeding in the Czech Republic in 2020 as two bodies conjoined into one. The external and internal structure of the body and the type of connection of both of the bodies are described. The weights of selected internal organs of the twins are compared with those of normally developed newborn guinea pigs.

The aim of this study was to test effects of age, gender, and sire line on dairy cattle behaviour. We have analyzed results of ethological tests for 40 Holstein breed animals (23 males and 17 females), offsprings of three sires. Maintenance behaviour were observed at the age of 90, 130 and 170 days. Behaviour in the maze was conducted at the age of 119 days, an open-field test was applied at the age of 124, 168, and 355 days. The social behaviour was determined by feeding on 155th day of the age. The times and the number of periods in all activities of maintenance behaviour were changing significantly (P<0.001) according to the age. The total time of lying, lying with ruminating, ruminating, feeding was increasing from the age of 90 days to the age of 170 days, on the other hand the time of standing was decreasing.The times of total lying, lying with ruminating, total ruminating, feeding were increased, and time of standing was decreased from the age of 90 days to the age of 170 days. Calves spent more time lying on the left side than on the right side. The number of ruminating periods was increasing according to the age. Eating periods were decreasing from the age of 90 to 170 days. The most of lying periods were recorded at the age of 130 days. The differences between sex were found in total time of lying, lying on the right side (P<0.05), and the males rest longer and had more periods of lying than females. We have found differences in times of feeding (P<0.001), total lying, standing (P<0.01), and lying on the left side (P<0.05) according to sire by comparing behaviour of the calves. Sire genotypes were significantly manifested in period number of total lying (P<0.001), lying on the right side, feeding (P<0.01), and standing (P<0.05). Males stood in the first part of maze longer than females (P<0.001), also length of total standing was longer by bulls (P<0.01). Heifers took shorter time to leave the maze than bulls (P<0.05). Sire lineages significantly differed in times of standing in the first part of maze and maze leaving. We did not find any significant differences either among sire lineage groups or between genders in locomotor behaviour measured by the number of crossed squares in open-field test. During all observations were more mobile heifers. No significant differences were found between males and females in social behaviour. The sire line influenced only the number of total duels (P<0.001). We found significant positive relationship between live body weight and time of staying in the first part of maze (r=0.3957**), time of maze leaving (r=0.3720**), and number of total and win duels (0.4031**, 0.3216*). Significant relationships were recorded in almost maintenance behaviour activities (P<0.001). Consistency of locomotor behaviour was proved only between the ages of 124 and 168 days (r=0.3177*). Significant relationship between maze behaviours and number of crossed squares were found only in ages of 119 and 124 days (r=-0.3721**; r=-0.4110**; r=-0.3994**).Keywords: cattle, growth, behaviour...

the aim of study was to find out the influence of milking by automatic milking system (AMS) on the physiological needs of dairy cows within 30 minutes after milking. Ethological study covered the needs of feed intake, water intake and need of lying down. The need of feed intake in 30 minutes after milking was found at 75.51 % of dairy cows in the barn with AMS. The need of water intake was found at 41.79 % of dairy cows. The percentage of cows, which needed to lie down after milking, was 3.7 %.