The influence of high-intensity ultrasound (HIU) on the technofunctional properties and structure of jackfruit seed protein isolate (JSPI) was investigated. Protein solutions (10%, w/v) were sonicated for 15min at 20kHz to the following levels of power output: 200, 400, and 600W (pulse duration: on-time, 5s; off-time 1s). Compared with untreated JSPI, HIU at 200W and 400W improved the oil holding capacity (OHC) and emulsifying capacity (EC), but the emulsifying activity (EA) and emulsion stability (ES) increased at 400W and 600W. The foaming capacity (FC) increased after all HIU treatments, as opposed to the water holding capacity (WHC), least gelation concentration (LGC), and foaming stability (FS), which all decreased except at pH 4 for FS. Tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) showed changes in the molecular weight of protein fractions after HIU treatment. Scanning electron microscopy (SEM) demonstrated that HIU disrupted the microstructure of JSPI, exhibiting larger aggregates. Surface hydrophobicity and protein solubility of the JSPI dispersions were enhanced after ultrasonication, which increased the destruction of internal hydrophobic interactions of protein molecules and accelerated the molecular motion of proteins to cause protein aggregation. These changes in the technofunctional and structural properties of JSPI could meet the complex needs of manufactured food products.
In the light of its functional properties found in this study, safflower protein isolate produced by ultrafiltration is recommended for use as an ingredient in food products such as salad dressing, meat products, mayonnaise, cakes, ice cream and desserts.
Physicochemical properties of eight samples of Mexican honeys from the West region were measured over 23 parameters, including moisture, water activity, soluble solids, hydroxymethylfurfural (HMF), ash, electrical conductivity, acidity (free, lactonic, and total), sugar content (fructose and glucose), color (L*, a*, b*), and mineral content. In addition, honey samples were classified according to the pollen quantitative analysis. According to the physicochemical parameters studied, the samples showed the values typical of honey. All honey samples were classified as dark according to the values of the L* color parameter. Only two honey samples were classified as monofloral and six as multifloral type. In general, the honey samples presented a good level of quality because they meet the physicochemical specifications of international regulation.
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