To compare the physical conditioning, hemodynamic, and salivary biomarkers between elderly athletes and the physically active elderly. 14 men: EA (n = 8) and PAE (n = 6). Collection times (T0; TE; T5; T15). A negative correlation was found between SF and cardiovascular parameters, BL, and STP in both groups, but this was almost double among PAE. For HR and SBP, there was a faster recovery in EA. The EA increase was correlated with SBP, while for PAE it was correlated with HR. BL showed an increase in TE, reaching 481% in EA and 639% in PAE. SNO showed a similar increase for the groups at the TE, but at T5, while EA already showed a reduction, PAE saw a 94% increase, with a slower decay for this group at T15. The SF presented the negative delta% was almost double in PAE, with a quick recovery already at T5 for EA and levels still negative at all times for PAE. For SIgA-s, there was an increase of 37% in EA and only 7% at the TE in PAE; 41% in EA and 15% in PAE for T5; and 26% in EA and 14% in PAE at T15. SA showed a higher peak in EA (TE) and less acute in PAE (T5) but there was a decrease among both at T15. STP increased by 126% in EA and 438% in PAE, already showing a return at T5 for EA, but increasing by 213% in PAE. Negative levels were reached at T15 for EA but levels remained high in PAE. Levels of physical conditioning affect cardiovascular parameters, salivary biomarkers, and their correlation within the over-60s.