Background: Bone marrow aspirate is a primary source for cell-based therapies with increasing value in the world of orthopaedic surgery, especially in revision cases of tendon and ligament repairs. However, cells within peritendinous structures, such as the paratenon and surrounding bursa, contribute to the native tendon-healing response and offer promising cell populations for cell-based repair strategies. Therefore, the purpose of this study is to investigate the efficacy of cells derived from human subacromial bursa as compared with the current gold standard, bone marrow stromal cells (BMSCs), for tendon repairs in an established in vivo immunodeficient murine patellar tendon defect model. Hypothesis: Subacromial bursal cells will show superior survival and engraftment into the host tissue as compared with BMSCs. Study Design: Controlled laboratory study. Methods: Human subacromial bursal and bone marrow aspirate were harvested from the same donor undergoing rotator cuff repair. Cells were transfected with a fluorescent lentiviral vector to permanently label the cells, encapsulated into fibrin gel, and implanted into bilateral full-length central-width patellar tendon defects of immunodeficient mice. Additional surgery was performed on control mice comparing fibrin without cells and natural healing. At the time of sacrifice, all limbs were scanned on a multiphoton microscope to monitor the engraftment of the human donor cells. Afterward, limbs were assigned to either immunohistochemical or biomechanical analysis. Results: As compared with BMSCs, implanted subacromial bursal cells displayed superior tissue engraftment and survival. The main healing response in this defect model was the creation of new healing tissue over the anterior surface of the defect space. The implantation of cells significantly increased the thickness of the anterior healing tissue as compared with control limbs that did not receive cells. Cell proliferation was also increased in limbs that received implanted cells, suggesting that the donor cells stimulated a more robust healing response. Finally, these changes in the healing response did not lead to significant changes in mechanical properties. Conclusion: The subacromial bursa, while often removed during rotator cuff repair, may harbor a more suitable cell source for tendon repair than BMSCs, as bursal cells display superior engraftment and survival in tendon tissue. In addition, the subacromial bursa may be a more accessible cell source than bone marrow aspirate. Clinical Relevance: The subacromial bursa contains a cell population that responds to tendon injury and may provide a more optimal cell source for tendon repair and regeneration strategies. Therefore, cells could be harvested from this tissue in the future, as opposed to the current practice of bursectomy and debridement.

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Background:Derotational osteotomy of the distal femur allows the anatomic treatment of patellofemoral maltracking due to increased femoral antetorsion. However, such rotational osteotomy procedures have a high potential of intended/unintended changes of frontal alignment.Purpose/Hypothesis:The purpose of this study was to perform derotational osteotomy of the distal femur and to demonstrate the utility of a novel trigonometric approach to address 3-dimensional (3D) changes on 2-dimensional imaging (axial computed tomography [CT] and frontal-plane radiography). The hypothesis was that 1-step single-cut osteotomy can simultaneously correct torsion and frontal alignment based on preoperatively calculated cutting angles.Study Design:Controlled laboratory study.Methods:Eight human cadaveric whole legs (4 lower limb torsos) underwent derotational osteotomy of the distal femur of 20°. A straight leg axis, determined as a mechanical femorotibial angle (mFTA) of 0°, was chosen as a goal for postoperative frontal alignment. The inclination of the cutting angle from the lateral view was calculated individually for each cadaveric leg and was represented by a simple 3D-printed cutting guide for surgery. Specimens underwent CT for the measurement of torsion, while the frontal leg axis was determined on an upright radiograph preoperatively and postoperatively. Preoperative and postoperative angles were compared with the mathematical prediction model.Results:The preoperative mFTA ranged from –3.9° (valgus) to +3.4° (varus) (mean, –0.2° ± 2.6°). A postoperative mean mFTA of 0.37° ± 0.69° (95% CI, –0.22° to 0.95°) was achieved (P = .01). Derotation showed a mean of 19.1° ± 2.1° (95% CI, 17.3°-20.8°). The oblique cutting plane for the correction of valgus legs showed a mean of 5.9° ± 6.8° and, for the correction of varus legs, a mean of –10.0° ± 4.5° projected on the perpendicular plane to the virtual anatomic shaft axis from the sagittal view.Conclusion:Single-cut distal femoral osteotomy can be performed to simultaneously address rotational as well as frontal alignment using a preoperatively defined oblique cut, as determined by the presented reproducible calculation model.Clinical Relevance:This study adds important knowledge to the technique of derotational osteotomy. This approach provides an individual, oblique single cut for the correction of torsion and frontal axis within a clinically insignificant margin. Simplified tables for calculation and a surgical reference make this model reproducible and safe.

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BackgroundDefining the optimal cutting plane for derotational osteotomy at the distal femur for correction of torsion in cases of patellofemoral instability is still challenging. This preliminary study investigates changes of frontal alignment by a simplified trigonometrical model and demonstrates a surgical guidance technique with the use of femur cadavers. The hypothesis was that regardless of midshaft bowing, a cutting plane perpendicular to the virtual anatomic shaft axis avoids unintended valgus malalignment due to derotation.MethodsA novel mathematical model, called the Pillar-Crane-Model, was developed to forecast changes on frontal alignment of the femur when a perpendicular cutting plane to the virtual anatomical shaft was chosen. As proof of concept, eight different torsion angles were assessed on two human cadaver femora (left and right). A single cut distal femoral osteotomy perpendicular to the virtual anatomical shaft was performed. Frontal plane alignment (mLDFA, aLDFA, AMA) was radiographically analyzed before and after rotation by 0°, 10°, 20°, and 30°. Measurements were compared to the model.ResultsThe trigonometrical equation from the Pillar-Crane-Model provides mathematical proof that slight changes into varus occur, seen by an increase in AMA and mLDFA, when the cutting plane is perpendicular to the virtual anatomical shaft axis. A table with standardized values is provided. Exemplarily, the specimens showed a mean increase of AMA from 4.8° to 6.3° and mLDFA from 85.2° to 86.7 after derotation by 30°. Throughout the derotation procedure, aLDFA remained at 80.4° ± 0.4°SD.ConclusionsWith the use of this model for surgical guidance and anatomic reference, unintended valgus changes on frontal malalignment can be avoided. When the cutting plane is considered to be perpendicular to the virtual anatomical shaft from a frontal and lateral view, a slight increase of mLDFA results when a derotational osteotomy of the distal femur is performed.