Modest dietary restriction (DR) prolongs life in a wide range of organisms, spanning single-celled yeast to mammals. Here, we report the use of recent techniques in nutrition research to quantify the detailed relationship between diet, nutrient intake, lifespan, and reproduction in Drosophila melanogaster. Caloric restriction (CR) was not responsible for extending lifespan in our experimental flies. Response surfaces for lifespan and fecundity were maximized at different protein-carbohydrate intakes, with longevity highest at a protein-to-carbohydrate ratio of 1:16 and egg-laying rate maximized at 1:2. Lifetime egg production, the measure closest to fitness, was maximized at an intermediate P:C ratio of 1:4. Flies offered a choice of complementary foods regulated intake to maximize lifetime egg production. The results indicate a role for both direct costs of reproduction and other deleterious consequences of ingesting high levels of protein. We unite a body of apparently conflicting work within a common framework and provide a platform for studying aging in all organisms.diet restriction ͉ geometric framework ͉ longevity ͉ nutrition ͉ aging A nimals that eat less live longer-up to a point. The view that dietary restriction without malnutrition prolongs life has become a central tenet in gerontology (1-3). Yeasts, fruit flies, nematode worms, and mice have become model systems for studying dietary restriction and aging, with some striking commonalities evident at the molecular and cellular levels (1-6). There is growing interest in the relationship between dietary restriction and quality and length of life in humans, although experimental data are lacking and difficult to obtain (7).It is widely held that the life-extending effects of dietary restriction (DR) are due to caloric restriction (CR) (1,8,9), but recently this view has been challenged by experiments suggesting that specific nutrients (proteins and certain amino acids) rather than energy are responsible (10-12). Claims and counterclaims in the debate over the roles of energy and specific nutrients in aging (8,(11)(12)(13)(14) have been hampered by one or more of the following problems (15). First, there is a lack of a suitable concept for baseline energy and nutrient intake, that is, dietary restriction relative to what? Thus, it may be that diet-restricted animals live longer either because eating less extends life, or because the normal nutritional regime in the laboratory is harmfully nutrient-rich in relation to requirements (15-17). Second, there have been too few dietary treatments used within an experiment to allow the effects of nutrients and energy to be partitioned. Hence, data from studies on rodents and Drosophila, which are claimed to prove the primacy of calories in influencing longevity, are open to alternative explanations (15). Finally, in the notable case of Drosophila, no study to date has measured how much flies actually eat throughout their lives. Rather, dietary restriction has been assumed to have occurred after dilution of the di...

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SummaryCaloric restriction (CR) has been widely accepted as a mechanism explaining increased lifespan (LS) in organisms subjected to dietary restriction (DR), but recent studies investigating the role of nutrients have challenged the role of CR in extending longevity. Fuelling this debate is the difficulty in experimentally disentangling CR and nutrient effects due to compensatory feeding (CF) behaviour. We quantified CF by measuring the volume of solution imbibed and determined how calories and nutrients influenced LS and fecundity in unmated females of the Queensland fruit fly, Bactocera tryoni (Diptera: Tephritidae). We restricted flies to one of 28 diets varying in carbohydrate:protein (C:P) ratios and concentrations. On imbalanced diets, flies overcame dietary dilutions, consuming similar caloric intakes for most dilutions. The response surface for LS revealed that increasing C:P ratio while keeping calories constant extended LS, with the maximum LS along C:P ratio of 21:1. In general, LS was reduced as caloric intake decreased. Lifetime egg production was maximized at a C:P ratio of 3:1. When given a choice of separate sucrose and yeast solutions, each at one of five concentrations (yielding 25 choice treatments), flies regulated their nutrient intake to match C:P ratio of 3:1. Our results (i) demonstrate that CF can overcome dietary dilutions; (ii) reveal difficulties with methods presenting fixed amounts of liquid diet; (iii) illustrate the need to measure intake to account for CF in DR studies and (iv) highlight nutrients rather than CR as a dominant influence on LS.

The distribution, systematics and ecology of Bactrocera tryoni, the Queensland fruit fly, are reviewed. Bactrocera tryoni is a member of the B. tryoni complex of species, which currently includes four named species, viz. B. tryoni ssp., B. neohumeralis, B. melas and B. aquilonis. The species status of B. melas and B. aquilonis is unclear (they may be junior synonyms of B. tryoni) and their validity, or otherwise, needs to be confirmed as a matter of urgency. While Queensland fruit fly is regarded as a tropical species, it cannot be assumed that its distribution will spread further south under climate change scenarios. Increasing aridity and hot dry summers, as well as more complex, indirect interactions resulting from elevated CO 2 , make predicting the future distribution and abundance of B. tryoni difficult. The ecology of B. tryoni is reviewed with respect to current control approaches (with the exception of sterile insect technique (SIT) which is covered in a companion paper). We conclude that there are major gaps in the knowledge required to implement most noninsecticide-based management approaches. Priority areas for future research include host-plant interactions, protein and cue-lure foraging and use, spatial dynamics, development of new monitoring tools, investigating the use of natural enemies and better integration of fruit flies into general horticultural IPM systems.Ann Appl Biol 158 (2011) 26-54

. Adult diet is an important determinant of sexual activity in many tephritid fruit flies. Whether availability of protein (hydrolysed yeast) in addition to sucrose influences sexual activity or longevity of male and female Queensland fruit flies ( Bactrocera tryoni Froggatt, 'Q-flies'), and whether irradiation of flies as pupae modifies their dietary needs, is investigated. Previous studies on groups of flies suggest that protein is required for sexual maturation of females but not males. By contrast, this study of individual flies demonstrates that protein in the adult diet provides a massive boost to sexual activity of both males and females. Mating probability increases with age from 4-14 days as the flies began to mature. However, mating probability reaches much higher levels when the flies are provided with protein. Although males and females mate at similar rates when provided with protein, females suffer a greater reduction in mating probability than males when deprived of protein.In addition to increased mating probability, access to dietary protein is also associated with reduced latency from onset of dusk until copulation. Furthermore, young male flies with access to dietary protein have longer copula duration than males fed only sucrose. Irradiation of flies as pupae has no apparent effect on mating probability, the latency to copulate or copula duration. However, when deprived of protein, sterile flies (especially males) suffer a greater reduction in longevity compared with fertile flies. Overall, access to dietary protein increases longevity for both males and females, although females live longer than males on both diets. These findings suggest that prerelease provision of dietary protein has the potential to greatly enhance the efficacy of Q-flies used in the sterile insect technique. & Lloyd, 1987;Vijaysegaran et al. , 1997 ). Under laboratory conditions, and in mass-reared cultures, protein is usually provided in the form of hydrolysed yeast ( Monro & Osborn, 1967;. found that although mixed sex groups of Q-flies fed just sucrose and water have very low fecundity, low fertility, and short lifespan, improvements in each of these measures can be achieved through protein-rich supplements of hydrolysed yeast or bacteria cultures. Drew (1987) later reported that, although groups of flies produce fertile offspring regardless of whether the males had received protein, they fail to produce eggs if females are denied protein. KeyThese studies indicate that protein is important for Q-fly populations and that there are sex differences in dependence on protein for production of fertile offspring. However, despite the clear trends in these studies of group fecundity and fertility, the effects of protein on individual mating performance remain to be investigated. Furthermore, there has not been any investigation of whether sterilization of flies for use in SIT has any influence on their protein requirements for mating. The present study builds upon the earlier work of Drew and colleagues by invest...