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This research investigated the feasibility of using constructed wetland system with vertical - subsurface flow (VF-SFS) for treating the decentralized domestic wastewater for household and small community. The pilot studied on three beds included three macrophytes tested parallel reed, vetiver and bulrush with four different hydraulic application rates (176, 132, 88 and 44 mm/ day).This study was tested with six different parameters such as biochemical oxygen demand (BOD), total suspended solids (TSS), orthophosphate (PO43-), ionized ammonia (NH4+) and nitrate (NO3-) nitrogen and total coliform loadings. The removal rate of characteristic parameters was obtained: BOD5 96%, TSS 96.9%, N-NH4+ 60.5%, PO43- 47.6%, total coliform 97.7%, respectively. In particular, the highest removal efficiency was obtained in the bed with the longer hydraulic retention time. Simultaneously, there was no removal difference among 03 beds constructed wetland with 03 different plant species, significantly. Through the results of research, VF-SFS can be considered an appropriate treatment method for domestic wastewater; however the additional pre-treatments were necessary to remove grit, heavy solids and floating scum.

Assessing the resistance, determining the nature of the resistance of the rice plant through the activity of three enzymes in rice: Catalase, Peroxydase and Phosphatase in the leaves of rice plants (the most active leaves of rice) rice plants) [2]. In addition, consider the role of Phosphatase in the fluctuations and the ability to accumulate P2O5 in soil and plants to see its role in rice's poison resistance in acid sulphate soil [4]. In other hand, Results shows that- High concentrations of toxins in the soil environment strongly inhibited the activity of Phosphatase enzymes in the roots, in the indicator leaves, and especially for rice, especially for plants that are less resistant to poisoning. But, Phosphatase enzyme in leaves has the effect on the providing energy for the plants to recover from poisoning. The activity of this enzyme is inversely correlated with the content of Al3+ in soil and Al3+ in plants. This proves that the antagonism between enzymes and toxins is evident, especially with Al3+ toxins. In other side, has highly toxic in the acid sulphate soil environment inhibits enzyme activity Peroxydase in rice roots. Under these conditions, peroxydase in the root plays a small role in helping the plant recover.We have sufficient basis to confirm that the enzyme Peroxydase in leaves plays an important role, against the harmful effects of toxins. When the toxin in the soil, in plants increased activity of this enzyme also increased higher than the control variant. Especially, when the toxicity increases, the Peroxydase enzyme in the rice leaves will also increase to resistant to toxicity ( + 280% (compared to the control); while the toxicty- non -resistant variety plants did not increase (compared to the control). When the toxicity decreased, Peroxydase activity in toxic-resistant plants was also higher than toxic non-resistant: This increased at + 643% for toxic-resistant rice compared to the control. While, in the weekly- resistant toxic- variety only increased + 314% (compared to the control).

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