From a fundamental standpoint, microbial electrochemistry is unravelling a thrilling link between life and materials. Technically, it may be the source of a large number of new processes such as microbial fuel cells for powering remote sensors, autonomous sensors, microbial electrolysers and equipment for effluent treatment. Microbial electron transfers are also involved in many natural processes such as biocorrosion. In these contexts, a huge number of studies have dealt with the impact of electrode materials, coatings and surface functionalizations but very few have focused on the effect of the surface topography, although it has often been pointed out as a key parameter impacting the performance of electroactive biofilms. The first part of the review gives an overview of the influence of electrode topography on abiotic electrochemical reactions. The second part recalls some basics of the effect of surface topography on bacterial adhesion and biofilm formation, in a broad domain reaching beyond the context of electroactivity. On these well-established bases, the effect of surface topography is reviewed and analysed in the field of electroactive biofilms. General trends are extracted and fundamental questions are pointed out, which should be addressed to boost future research endeavours. The objective is to provide basic guidelines useful to the widest possible range of research communities so that they can exploit surface topography as a powerful lever to improve, or to mitigate in the case of biocorrosion for instance, the performance of electrode/biofilm interfaces.

Smooth and nano-rough flat gold electrodes were manufactured with controlled Ra of 0.8 and 4.5nm, respectively. Further nano-rough surfaces (Ra 4.5nm) were patterned with arrays of micro-pillars 500μm high. All these electrodes were implemented in pure cultures of Geobacter sulfurreducens, under a constant potential of 0.1V/SCE and with a single addition of acetate 10mM to check the early formation of microbial anodes. The flat smooth electrodes produced an average current density of 0.9A·m. The flat nano-rough electrodes reached 2.5A·m on average, but with a large experimental deviation of ±2.0A·m. This large deviation was due to the erratic colonization of the surface but, when settled on the surface, the cells displayed current density that was directly correlated to the biofilm coverage ratio. The micro-pillars considerably improved the experimental reproducibility by offering the cells a quieter environment, facilitating biofilm development. Current densities of up to 8.5A·m (per projected surface area) were thus reached, in spite of rate limitation due to the mass transport of the buffering species, as demonstrated by numerical modelling. Nano-roughness combined with micro-structuring increased current density by a factor close to 10 with respect to the smooth flat surface.