New types of devices made using thin‐film‐transistor (TFT) technology were used in this article for dielectrophoresis purpose with microbeads, yeast cells, and HepG2 cells. That technology allows obtaining centimeter size transparent substrates covered with a large and dense array of 100 µm microelectrodes individually controllable. The purpose of these devices is the development of platforms for various applications on cells and cells culture, like manipulation, sensing, monitoring, or stimulation on the same device. Dielectrophoresis is one of the applications and is a key target for precise patterning and 2D displacement of cells on the device. In this article, 2D results of dielectrophoresis with microbeads and yeast cells and device programming are described. Then, application to mammal HepG2 liver cells shows the possibility of cells patterning with such devices. © 2019 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.

The constant development and improvement of microelectromechanical systems (MEMS) have been given a great opportunity to develop new reliable microsystems devices for biomedical research. This article presents a locally addressable 2-D arrayed indium-tin oxide microelectrode platform with integrated thin-film transistors (TFTs) for biological and chemical sensing. Microelectrode arrays (MEAs) using TFT have the advantage of being transparent with a high density of microelectrodes on a large surface. In vitro 2-D electrical measurements on 28 parallel-connected lines selected from a 22 500 MEA were successfully performed with heart cells for the first time. Observation of cell contraction was performed simultaneously with an inverted microscope. Voltammetry and amperometry measurements were also demonstrated using the transparent microelectrodes as working electrodes and an integrated Ag/AgCl reference electrode. Due to its unique features, we believe that the TFT platform can provide more understanding of the key communication between heart cells, and large-scale cardiovascular and nervous systems. As a result, the platform offers unique access to versatile Manuscript

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