The effect of drying temperature (60, 100, and 140 °C) on the
polyphenols' content and antioxidant
activity of red grape pomace peels was studied. Freeze-dried
samples were used as reference.
Differences on the CIE-LAB color, total extractable polyphenols,
condensed tannins, UV−vis spectra,
and antioxidant activity were evaluated. When drying temperature
was 100 and 140 °C, a significant
reduction in both total extractable polyphenols (18.6 and 32.6%) and
condensed tannins (11.1 and
16.6%) was observed, as well as a decrease of 28 and 50% in the
antioxidant activity of the samples,
respectively. Hue angle and total color difference in the sample
dried at 140 °C were significantly
higher than in the freeze-dried reference material. A red color
loss at 140 °C was also confirmed by
lower absorbance values in the spectra at 525 nm. Drying at 60
°C did not significantly affect the
sample characteristics evaluated.
Keywords: Wine byproducts; grape pomace peels; antioxidant activity; drying
temperature
Fucus vesiculosus was sequentially extracted with water at 22 degrees C (fraction 1 (F1)) and 60 degrees C (F2), and with 0.1 M HCl (F3) and 2 M KOH (F4) at 37 degrees C. Soluble fractions (42.3% yield) were composed of neutral sugars (18.9-48 g/100 g), uronic acids (8.8-52.8 g/100 g), sulfate (2.4-11.5 g/100 g), small amounts of protein (< 1-6.1 g/100 g), and nondialyzable polyphenols (0.1-2.7 g/100 g). The main neutral sugars were fucose, glucose, galactose, and xylose. Infrared (IR) spectra of the fractions showed absorption bands at 820-850 and 1225-1250 cm(-1) for sulfate. F1, F2, and F4 also exhibited an absorption band at 1425 cm(-1), due to uronic acids, and their IR spectra resembled that of alginate. F3 had an IR spectrum similar to that of fucoidan with an average molecular weight of 1.6 x 10(6) Da, calculated by molecular exclusion high-performance liquid chromatography. The presence of fucose in this polysaccharide was confirmed by (1)H NMR spectroscopy. This fraction showed the highest potential to be antioxidant by the ferric reducing antioxidant power (FRAP) assay, followed by the alkali- and water-soluble fractions. Sulfated polysaccharides from edible seaweeds potentially could be used as natural antioxidants by the food industry.
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