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Objective: Although anemia is frequently seen in children, there isn't adequate number of study related with this issue. In this study, we aimed to investigate iron, folate and vitamin B12 status in children and adolescents in a single center in the Aegean region of our country. Method: We retrospectively evaluated the children without chronic disease who were admitted to our hospital between 2008-2016 (n=7310). Ferritin, iron and iron binding capacity were measured by Olympus 2700 analyzer. Folate and vitamin B12 were measured using Immulite 2000 and Cobas E411 analyzer, respectively. MCV and hemoglobin were measured with Beckman Coulter LH750. Kruskal-Wallis and Mann-Whitney U tests were used for comparison between groups. Results: Children (2743 boys, and 4567 girls) aged between 1-18 years old were screened. The percentage of iron, folate, vitamin B12 and combined iron and vitamin B12 deficiencies were found to be 21.7%, 8%, 16.9%, and 4.7% respectively, while 18.8% of all children were in the anemic group. The incidence of combined iron and vitamin B12 in anemic group was found to be high (10.2%), although anemic and nonanemic groups were similar with respect to vitamin B12 and folate deficiencies. Conclusion: Iron deficiency may mask the megaloblastic anemia caused by vitamin B12 and folate deficiency. Effective preventive social nutrition programs may be useful in preventing anemia.

Although we found values different from the limits stated in the kit insert, it would be better to confirm our findings with the direct method, especially in APTT for patients under the age of 40 and over the age of 59, and also for sex differences in PT.

ÖZAmaç: Laboratuvardaki toplam hataların büyük çoğunluğunu preanalitik evre hataları oluşturur (%46-68). Bu çalışmanın amacı, acil laboratuvarımızdaki bazı test gruplarına ilişkin reddedilen örneklerin oranlarını ve nedenlerini araştırmaktı. Yöntem: Çalışma Kasım 2014 ve Nisan 2015 tarihleri arasında reddedilen örnekler üzerine planlandı ve acil laboratuvarındaki 6 test grubu (klinik kimya, ilaç düzeyi, kardiyak belirteçler, koagülasyon, eritrosit sedimantasyon hızı, tam idrar tetkiki) dâhil edildi. Numunelerin toplam sayısı ve reddedilen örnekler, hastane bilgi sisteminden retrospektif olarak elde edildi. Hatalar test grubuna, örnek tipine ve ret nedenlerine göre sınıflandırıldı. Bulgular: Altı aylık dönemde acil laboratuvarımıza toplam 144387 numune geldiği belirlendi. Numunelerin %1,14'ü preanalitik hatalar nedeniyle reddedildi. Yanlış kodlama en sık ret nedeni olarak bulundu (%22,9). Fazla kodlama, hemoliz ve yetersiz numune ret oranları ise sırasıyla %20,2, %17, %12,5 olarak belirlendi. Sonuç: Çalışmamızda preanalitik hataların en sık nedeni test istem hataları idi. Tıbbi sekreterlerin eğitimi bu sorunu çözüme kavuşturulabilir.Anahtar kelimeler: Preanalitik değişkenler, numunelerin reddedilmesi, laboratuvar hataları, klinik laboratuvar ABSTRACT Objective: Preanalytical phase errors constitute the majority of the total number of errors in the laboratory (46-68%). The aim of this study was to investigate the causes and rates of rejected samples in certain test groups in our emergecy laboratory. Methods: This study was designed on the samples rejected between November 2014 and April 2015 and six test groups examined in emergecy laboratory (clinical chemistry, drug level, cardiac markers, coagulation, erythrocyte sedimentation rate, complete urinalysis) were included in the study. The total number of specimens and rejected samples were obtained from the Hospital Information System retrospectively. Laboratory errors were classified by test groups, specimen types and causes of specimen rejection. Results: The study was planned on 144887 blood samples sent to our emergency laboratory within six months between November 2014 and April 2015 and 1.14% of them were rejected because of preanalytical errors. Erroneous coding was found as the most common cause of rejection (22.9%). Rejection rates for extraneous coding, hemolysis and insufficient volume were found to be 20.2%, 17% and 12.5%, respectively. Conclusions: In our study, test request errors were the most common causes of preanalytical errors. Education of medical secretaries can solve this problem.

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