Understanding how ecological interactions affect vector-borne disease dynamics is crucial in the context of rapid biodiversity loss and increased emerging vector-borne diseases. Although there have been many studies on the impact of interspecific competition and host competence on disease dynamics, few of them have addressed the case of a vector-borne disease. Using a simple compartment model with two competing host species and one vector, we investigated the combined effects of vector preference, host competence, and interspecific competition on disease risk in a vector-borne system. Our research demonstrated that disease transmission dynamics in multi-host communities are more complex than anticipated. Vector preference and differences in host competence shifted the direction of the effect of competition on community disease risk, yet interspecific competition quantitatively but not qualitatively changed the effect of vector preference on disease risk. Our work also identified the conditions of the dilution effect and amplification effect in frequency-dependent transmission mode, and we discovered that adding vector preference and interspecific competition into a simple two-host-one-vector model altered the outcomes of how increasing species richness affects disease risk. Our work explains some of the variation in outcomes in previous empirical and theoretical studies on the dilution effect.

Rapid global biodiversity loss and increasing emerging infectious diseases underscore the significance of identifying the diversity-disease relationship. Although experimental evidence supports the existence of dilution effects in several natural ecosystems, we still know very little about the conditions under which a dilution effect will occur. Using a multi-host Susceptible-Infected-Recovered model, we found when disease transmission was density-dependent, the diversity-disease relationship could exhibit an increasing, decreasing, or non-monotonic trend, which mainly depended on the patterns of community assembly. However, the combined effects of the host competence-abundance relationship and species extinction order may reverse or weaken this trend. In contrast, when disease transmission was frequency-dependent, the diversity-disease relationship only showed a decreasing trend, the host competence-abundance relationship and species extinction order did not alter this decreasing trend, but it could reduce the detectability of the dilution effect and affect disease prevalence. Overall, a combination of disease transmission mode, community assembly pattern, and host community composition determines the direction or strength of the diversity-disease relationship. Our work helps explain why previous studies came to different conclusions about the diversity-disease relationship and provides a deeper understanding of the pathogen transmission dynamics in actual communities.