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To demonstrate the influence of high-quality nursing intervention on bad mood, postoperative pain and quality of life (QOL) of patients undergoing gallstone surgery. One hundred and thirty-three patients with gallstone surgery in our hospital were selected as the research participants, of which 76 cases received high-quality nursing during hospitalization and were regarded as the research group (RG), and the remaining 57 cases received routine nursing care alone and were included in the control group (CG). The two series were compared regarding pain status, postoperative recovery, pain scores, pain duration and frequency pre- and post-intervention, incidence of complications, psychological state scores pre-and post-intervention, as well as nursing satisfaction and postoperative QOL.The number of patients with pain in RG was notably less than that in CG (P < 0.001). RG presented shorter anal exhaust time, ambulation time and hospitalization timethan CG (P < 0.05). Also, in comparison with CG, RG displayed lower pain scores post intervention (P < 0.05), with shorter pain duration and pain frequency (P < 0.05). RG had fewer complications than CG (P < 0.009). The scores of depression and anxiety were notably lower in RG than in CG (P < 0.05). RG showed higher satisfaction and recognition than CG (P < 0.001). The QOL of patients was also superior in RG (P < 0.05).High-quality nursing intervention can validly mitigate postoperative bad mood, reduce postoperative pain and meliorate postoperative QOL of patients with gallstone surgery.