Cultivating algae on various stillage types present an alternative to current stillage application for fertirrigation of the sugarcane. The objective of this study was to determine how algal concentration, nutrient removal efficiency and elemental composition of Desmodesmus sp. algae change in response to different (raw and digested) types of stillage. Algal biomass was cultivated and harvested at laboratory scale. Obtained concentrations were 0.5 g/L and 0.3 g/L for Desmodesmus sp. grown in raw and digested stillage, respectively. At these concentrations, total carbon (TC) and total nitrogen (TN as Total Kjeldahl Nitrogen or N-TKN) removal from stillage medium accounted 39.78% and 5.58% for raw; 20.83% and 5.43% for digested stillage, respectively. The Desmodesmus sp. used in the present study contained high carbon and oxygen content, similar to other algal species. Calcium, phosphorus, and sulfur were the three most abundant inorganic elements. Both biomasses were free of chlorine and with the low content of potassium. The phosphorous content in both biomasses was in the range of 6.03%-6.84%, which could classify these biomasses as useful plant nutrients.